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NeuN Mouse mAb (A26951)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - NeuN Mouse mAb (A26951)

Western blot analysis of various lysates using NeuN Mouse mAb (A26951) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - NeuN Mouse mAb (A26951)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using NeuN Mouse mAb (A26951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NeuN Mouse mAb (A26951)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using NeuN Mouse mAb (A26951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - NeuN Mouse mAb (A26951)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NeuN Mouse mAb (A26951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - NeuN Mouse mAb (A26951)

Confocal imaging of paraffin-embedded Human brain tissue using NeuN Mouse mAb (A26951, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - NeuN Mouse mAb (A26951)

Confocal imaging of paraffin-embedded Mouse brain tissue using NeuN Mouse mAb (A26951, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Immunofluorescence - NeuN Mouse mAb (A26951)

Confocal imaging of paraffin-embedded Rat brain tissue using NeuN Mouse mAb (A26951, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameNeuN Mouse mAb
Catalog No.A26951
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
CloneNo.AMC50043
This gene encodes a member of the RNA-binding FOX protein family which is involved in the regulation of alternative splicing of pre-mRNA. The protein has an N-terminal proline-rich region, an RNA recognition motif (RRM) domain, and a C-terminal alanine-rich region. This gene produces the neuronal nuclei (NeuN) antigen that has been widely used as a marker for post-mitotic neurons. This gene has its highest expression in the central nervous system and plays a prominent role in neural tissue development and regulation of adult brain function. Mutations in this gene have been associated with numerous neurological disorders. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms.
ImmunogenRecombinant protein of human NeuN.
SequenceEmail for sequence
Gene ID
Swiss Prot
SynonymsFOX3; NEUN; FOX-3; HRNBP3
Calculated MW34kDa
Observed MW46-55kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:2500 - 1:5000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationCytoplasm, Nucleus.

Documents

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ABclonal:Western blot - NeuN Mouse mAb (A26951)}

Western blot - NeuN Mouse mAb (A26951)

Western blot analysis of various lysates using NeuN Mouse mAb (A26951) at 1:5000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - NeuN Mouse mAb (A26951)}

Immunohistochemistry - NeuN Mouse mAb (A26951)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using NeuN Mouse mAb (A26951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NeuN Mouse mAb (A26951)}

Immunohistochemistry - NeuN Mouse mAb (A26951)

Immunohistochemistry analysis of paraffin-embedded Human brain tissue using NeuN Mouse mAb (A26951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - NeuN Mouse mAb (A26951)}

Immunohistochemistry - NeuN Mouse mAb (A26951)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NeuN Mouse mAb (A26951) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - NeuN Mouse mAb (A26951)}

Immunofluorescence - NeuN Mouse mAb (A26951)

Confocal imaging of paraffin-embedded Human brain tissue using NeuN Mouse mAb (A26951, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - NeuN Mouse mAb (A26951)}

Immunofluorescence - NeuN Mouse mAb (A26951)

Confocal imaging of paraffin-embedded Mouse brain tissue using NeuN Mouse mAb (A26951, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
ABclonal:Immunofluorescence - NeuN Mouse mAb (A26951)}

Immunofluorescence - NeuN Mouse mAb (A26951)

Confocal imaging of paraffin-embedded Rat brain tissue using NeuN Mouse mAb (A26951, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.