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ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Rabbit

ABclonal:Flow CytoMetry - ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159)

Flow cytometry: 1X10^6 RK13 cells (negative control, left) and Rabbit PBMC (right) were surface-stained with ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159, 5 μl/Test, orange line) or ABflo® 647 Mouse IgG1 isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159)

Flow cytometry: 1X10^6 Rabbit PBMC were surface-stained with ABflo® 647 Mouse IgG1 isotype control (5 μl/Test, left) or ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159, 5 μl/Test, right).

Overview

Product nameABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb
Catalog No.A25159
Host speciesMouse
Purification methodAffinity purification
IsotypeMouse IgG1
CloneNo.AMC50004-ABf647
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 107-458 of rabbit IgM (Fc) (P03988).
Sequence VDSELPPNVSVFIPPRDSFSGSGTRKSRLICQATGFSPKQISVSWLRDGQKVESGVLTKPVEAETKGAGPATFSISSMLTITESDWLSQSLYTCRVDHRGIFFDKNVSMSSECSTTPSPGIQVFPIAPSFADTFLSKSARLICLVTDLTTYGSLNISWASHNGKALDTHMNITESHPNATFSAMGEASVCAEDWESGEQFTCTVTHADLPFPLKHTISKSREVAKHPPAVYVLPPAREQLVLRESATVTCLVKGFSPADVFVQWQQRGQPLSSDKYVTSAPAPEPQAPGLYFTHSTLTVTEEDWNSGETFTCVVGHEALPHMVTERTVDKSTGKPTLYNVSLIMSDTASTCY
Gene ID
Swiss Prot
Synonyms
Calculated MW52kDa
Observed MW
ReactivityRabbit
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationSecreted

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159)}

    Flow CytoMetry - ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159)

    Flow cytometry: 1X10^6 RK13 cells (negative control, left) and Rabbit PBMC (right) were surface-stained with ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159, 5 μl/Test, orange line) or ABflo® 647 Mouse IgG1 isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159)}

    Flow CytoMetry - ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159)

    Flow cytometry: 1X10^6 Rabbit PBMC were surface-stained with ABflo® 647 Mouse IgG1 isotype control (5 μl/Test, left) or ABflo® 647 Mouse Anti-Rabbit IgM (Fc) mAb (A25159, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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