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pan Phospho-Serine/Threonine Mouse mAb (AP1067)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

Western blot analysis of lysates from A-431 cells using pan Phospho-Serine/Threonine Mouse mAb (AP1067) at 1:1000 dilution incubated overnight at 4℃. A-431 cells were treated by Calyculin A (50 nM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 30 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - pan Phospho-Serine/Threonine Mouse mAb (AP1067)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue, untreated (left) and lambda phosphatase-treated (right), using pan Phospho-Serine/Threonine Mouse mAb (AP1067) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue, untreated (left) and lambda phosphatase-treated (right), using pan Phospho-Serine/Threonine Mouse mAb (AP1067) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue, untreated (left) and lambda phosphatase-treated (right), using pan Phospho-Serine/Threonine Mouse mAb (AP1067) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Overview

Product name	pan Phospho-Serine/Threonine Mouse mAb
Catalog No.	AP1067
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG2b, kappa
CloneNo.	AMC0265

Background

Protein phosphorylation is one of the main key regulatory mechanisms by which extracellular signals are conveyed. Global alteration of signal transduction by inhibition of serine/threonine dephosphorylation has recently been shown to markedly potentiate cancer cell killing by the DNA-methylating drug, temozolomide.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence containing phosphorylated Serine/Threonine.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW	＞10kDa

Applications

Reactivity	Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:1000 - 1:5000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	A-431 treated by Calyculin A
Cellular location
Customer validation	WB(Mus musculus, Other, Homo sapiens) IF(Mus musculus) IHC(Mus musculus) RT-qPCR(Mus musculus) WB(Mus musculus)