제품 > 항체 > 단일클론항체(mAb)

Human Kappa Light Chain Rabbit mAb (A23689)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from Human serum, using Human Kappa Light Chain Rabbit mAb (A23689) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.5s.

ABclonal:Immunofluorescence - Human Kappa Light Chain Rabbit mAb (A23689)

Immunofluorescence analysis of paraffin-embedded human tonsil using Human Kappa Light Chain Rabbit mAb (A23689) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - Human Kappa Light Chain Rabbit mAb (A23689)

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or Human Immunoglobulin kappa constant mAb(A23689, 2 μg/mL, right).

Overview

Product name	Human Kappa Light Chain Rabbit mAb
Catalog No.	A23689
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60646

Background

Immunoglobulins belong to a group of related glyco proteins which make up 20% of serum proteins. Antigens and immunoglobulins react to confer immunity to individuals. Immunoglobulins have similar structures of two identical heavy chains and two identical light chains. Both the heavy chains and the light chains are divided into constant and variable regions. The constant regions have the same amino acid sequences between all the immunoglobulin classes. The variable regions have approximately 110 amino acids with high sequence variability. The amino acid sequence of the heavy chain determines the class of an immunoglobulin. The five types of immunoglobulin heavy chains are known as: IgG, IgA, IgM, IgD, and IgE. IgG is divided into four subclasses, and IgA is divided into two subclasses. In serum IgA and IgG are monomers with a single 4 polypeptide unit; while, IgM is a pen tamer. IgA may also form polymers. Kappa light chain antibody can be used for the identification of leukemias, plasmacytomas and certain non Hodgkin's lymphomas. Kappa light chain contains one immunoglobulin like domain.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-107 of human Kappa Light Chain(P01834).
Sequence	RTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGEC
Gene ID
Swiss Prot
Synonyms	Km; HCAK1; IGKCD; Human Kappa Light Chain
Calculated MW	12kDa
Observed MW	25kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 FC 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Flow Cytometry
Positive samples	Human serum
Cellular location	Cytoplasmic, Secreted

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Human Kappa Light Chain Rabbit mAb images

Western blot - Human Kappa Light Chain Rabbit mAb (A23689)

Immunofluorescence - Human Kappa Light Chain Rabbit mAb (A23689)

Flow CytoMetry - Human Kappa Light Chain Rabbit mAb (A23689)

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or Human Immunoglobulin kappa constant mAb(A23689, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.