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APC Mouse anti-Human CD11b mAb (A26893)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 Human peripheral blood granulocytes were surface-stained with APC Mouse anti-Human CD11b mAb (A26893, 5 μl/Test, orange line) or APC Mouse IgG2b isotype control (A22884, 5μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).Cells in the granulocytes gate were used for analysis.

Flow cytometry: 1X10^6 Human peripheral blood granulocytes were surface-stained with APC Mouse IgG2b isotype control (A22884, 5 μl/Test, left) or APC Mouse anti-Human CD11b mAb (A26893, 5 μl/Test, right).Cells in the granulocytes gate were used for analysis.

Flow cytometry: 1X10^6 Human peripheral blood granulocytes were surface-stained with ABflo® 488 Rabbit anti-Human CD62L/L-Selectin mAb (A23020, 5 μl/Test) and APC Mouse IgG2b isotype control (A22884, 5 μl/Test, left) or APC Mouse anti-Human CD11b mAb (A26893, 5 μl/Test, right). Cells in the granulocytes gate were used for analysis.

Overview

Product name	APC Mouse anti-Human CD11b mAb
Catalog No.	A26893
Host species	Mouse
Purification method	Affinity purification
Isotype	IgG
CloneNo.	AMC0709-APC

Background

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant protein of human CD11b.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6
Calculated MW	127kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Membrane, Single-pass type I membrane protein.