제품 > 항체 > 다중클론항체(pAb)

67kDa Laminin Receptor Rabbit pAb (A9008)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - 67kDa Laminin Receptor Rabbit pAb (A9008)

Western blot analysis of various lysates using 67kDa Laminin Receptor Rabbit pAb (A9008) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - 67kDa Laminin Receptor Rabbit pAb (A9008)

Western blot analysis of various lysates using 67kDa Laminin Receptor Rabbit pAb (A9008) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunofluorescence - 67kDa Laminin Receptor Rabbit pAb (A9008)

Immunofluorescence analysis of L929 cells using 67kDa Laminin Receptor Rabbit pAb (A9008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - 67kDa Laminin Receptor Rabbit pAb (A9008)

Immunofluorescence analysis of U-2 OS cells using 67kDa Laminin Receptor Rabbit pAb (A9008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name67kDa Laminin Receptor Rabbit pAb
Catalog No.A9008
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Many of the effects of laminin are mediated through interactions with cell surface receptors. These receptors include members of the integrin family, as well as non-integrin laminin-binding proteins. This gene encodes a high-affinity, non-integrin family, laminin receptor 1. This receptor has been variously called 67 kD laminin receptor, 37 kD laminin receptor precursor (37LRP) and p40 ribosome-associated protein. The amino acid sequence of laminin receptor 1 is highly conserved through evolution, suggesting a key biological function. It has been observed that the level of the laminin receptor transcript is higher in colon carcinoma tissue and lung cancer cell line than their normal counterparts. Also, there is a correlation between the upregulation of this polypeptide in cancer cells and their invasive and metastatic phenotype. Multiple copies of this gene exist, however, most of them are pseudogenes thought to have arisen from retropositional events. Two alternatively spliced transcript variants encoding the same protein have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human 67kDa Laminin Receptor (NP_002286.2).
SequenceMSGALDVLQMKEEDVLKFLAAGTHLGGTNLDFQMEQYIYKRKSDGIYIINLKRTWEKLLLAARAIVAIENPADVSVISSRNTGQRAVLKFAAATGATPIAGRFTPGTFTNQIQAAFREPRLLVVTDPRADHQPLTEASYVNLPTIALCNTDSPLRYVDIAIPCNNKGAHSVGLMWWMLAREVLRMRGTISREHPWEVMPDLYFYRDPEEIEKEEQAAAEKAVTKEEFQGEWTAPAPEFTATQPEVADWSEGVQVPSVPIQQFPTEDWSAQPATEDWSAAPTAQATEWVGATTDWS
Gene ID
Swiss Prot
SynonymsSA; LBP; LRP; p40; uS2; 67LR; ICAS; lamR; 37LRP; LAMBR; LAMR1; LRP/LR; LBP/p40; NEM/1CHD4; 67kDa Laminin Receptor
Calculated MW33kDa
Observed MW37kDa/67kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, HeLa, B-cell, Mouse heart, Mouse kidney, Rat liver
Cellular locationCell membrane, Cytoplasm, Nucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - 67kDa Laminin Receptor Rabbit pAb (A9008)}

Western blot - 67kDa Laminin Receptor Rabbit pAb (A9008)

Western blot analysis of various lysates using 67kDa Laminin Receptor Rabbit pAb (A9008) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - 67kDa Laminin Receptor Rabbit pAb (A9008)}

Western blot - 67kDa Laminin Receptor Rabbit pAb (A9008)

Western blot analysis of various lysates using 67kDa Laminin Receptor Rabbit pAb (A9008) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunofluorescence - 67kDa Laminin Receptor Rabbit pAb (A9008)}

Immunofluorescence - 67kDa Laminin Receptor Rabbit pAb (A9008)

Immunofluorescence analysis of L929 cells using 67kDa Laminin Receptor Rabbit pAb (A9008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - 67kDa Laminin Receptor Rabbit pAb (A9008)}

Immunofluorescence - 67kDa Laminin Receptor Rabbit pAb (A9008)

Immunofluorescence analysis of U-2 OS cells using 67kDa Laminin Receptor Rabbit pAb (A9008) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)