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ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191)

Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and HEL cells (right) were surface-stained with Mouse anti-Human CD235ab mAb (IgG2b, κ, orange line) or Mouse IgG2b isotype control (2 μg/mL, blue line), followed by ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191, 5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191)

Flow cytometry: 1X10^6 HEL cells were surface-stained with Mouse IgG2b isotype control (2 μg/mL, left) or Mouse anti-Human CD235ab mAb (IgG2b, κ, right). ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191, 5 μl/Test) was used as a secondary antibody.

Overview

Product nameABflo® 488 Rabbit Anti-Mouse IgG2b mAb
Catalog No.A26191
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC67783-ABflo488
Enables antigen binding activity. Acts upstream of or within phagocytosis; positive regulation of immune response; and positive regulation of phagocytosis. Located in extracellular space. Part of immunoglobulin complex, circulating. Orthologous to several human genes including IGHG1 (immunoglobulin heavy constant gamma 1 (G1m marker)); IGHG2 (immunoglobulin heavy constant gamma 2 (G2m marker)); and IGHG3 (immunoglobulin heavy constant gamma 3 (G3m marker)).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 14-113 of mouse IgG2b (P01867-2).
SequenceCGDTTGSSVTLGCLVKGYFPESVTVTWNSGSLSSSVHTFPALLQSGLYTMSSSVTVPSSTWPSQTVTCSVAHPASSTTVDKKLEPSGPISTINPCPPCKE
Gene ID
Swiss Prot
SynonymsIgG2b; Igh-3; gamma2b
Calculated MW37kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Secreted.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191)}

    Flow CytoMetry - ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191)

    Flow cytometry: 1X10^6 Jurkat cells (negative control, left) and HEL cells (right) were surface-stained with Mouse anti-Human CD235ab mAb (IgG2b, κ, orange line) or Mouse IgG2b isotype control (2 μg/mL, blue line), followed by ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191, 5 μl/Test) staining. Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191)}

    Flow CytoMetry - ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191)

    Flow cytometry: 1X10^6 HEL cells were surface-stained with Mouse IgG2b isotype control (2 μg/mL, left) or Mouse anti-Human CD235ab mAb (IgG2b, κ, right). ABflo® 488 Rabbit Anti-Mouse IgG2b mAb (A26191, 5 μl/Test) was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.

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