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ABflo® 488 Rabbit anti-Human IgE mAb (A23696)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgE mAb (A23696)

Flow cytometry:1X10^6 HeLa cells(negative control, left) and Human PBMC(right) were surface-stained with ABflo® 488 Rabbit anti-Human IgE mAb(A23696, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Overview

Product nameABflo® 488 Rabbit anti-Human IgE mAb
Catalog No.A23696
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60066-ABf488
IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 104-428 of human IgE (P01854).
SequenceVKILQSSCDGGGHFPPTIQLLCLVSGYTPGTINITWLEDGQVMDVDLSTASTTQEGELASTQSELTLSQKHWLSDRTYTCQVTYQGHTFEDSTKKCADSNPRGVSAYLSRPSPFDLFIRKSPTITCLVVDLAPSKGTVNLTWSRASGKPVNHSTRKEEKQRNGTLTVTSTLPVGTRDWIEGETYQCRVTHPHLPRALMRSTTKTSGPRAAPEVYAFATPEWPGSRDKRTLACLIQNFMPEDISVQWLHNEVQLPDARHSTTQPRKTKGSGFFVFSRLEVTRAEWEQKDEFICRAVHEAASPSQTVQRAVSVNPGL
Gene ID
Swiss Prot
SynonymsIgE
Calculated MW47kDa/54kDa/59kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationExtracellular region, extracellular space, plasma membrane.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgE mAb (A23696)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Human IgE mAb (A23696)

    Flow cytometry:1X10^6 HeLa cells(negative control, left) and Human PBMC(right) were surface-stained with ABflo® 488 Rabbit anti-Human IgE mAb(A23696, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)