제품 > 항체 > 단일클론항체(mAb)

ABflo® 594 Rabbit anti-Human IFN-alpha/beta R2 mAb (A25114)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 T-47D cells (Low Expression, left) and U266 cells (right) were surface-stained with ABflo® 594 Rabbit anti-Human IFN-alpha/beta R2 mAb (A25114, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 U266 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human IFN-alpha/beta R2 mAb (A25114, 5 μl/Test, right).

Overview

Product name	ABflo® 594 Rabbit anti-Human IFN-alpha/beta R2 mAb
Catalog No.	A25114
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63166-ABflo594

Background

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The protein belongs to the type II cytokine receptor family. Mutations in this gene are associated with Immunodeficiency 45.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 28-237 of human IFN-alpha/beta R2(NP_008896.2).
Sequence	SYDSPDYTDESCTFKISLRNFRSILSWELKNHSIVPTHYTLLYTIMSKPEDLKVVKNCANTTRSFCDLTDEWRSTHEAYVTVLEGFSGNTTLFSCSHNFWLAIDMSFEPPEFEIVGFTNHINVMVKFPSIVEEELQFDLSLVIEEQSEGIVKKHKPEIKGNMSGNFTYIIDKLIPNTNYCVSVYLEHSDEQAVIKSPLKCTLLPPGQESE
Gene ID
Swiss Prot
Synonyms	IFN-R; IMD45; IFNABR; IFNARB; IFN-R-2; IFN-alpha-REC; IFNAR2
Calculated MW	58kDa/37kDa/27kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Membrane, Secreted, Single-pass type I membrane protein