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ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)

Flow cytometry: 1X10^6 HAP1 cells (negative control, left) and NK-92MI cells (right) were surface-stained with ABflo® 610 Rabbit anti-Human CD94 mAb (A26683, 5 μl/Test, orange line) or ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)

Flow cytometry: 1X10^6 NK-92MI cells were surface-stained with ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, left) or ABflo® 610 Rabbit anti-Human CD94 mAb (A26683, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 610 Rabbit anti-Human CD94 mAb (A26683, 5 μl/Test, orange line) or ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).Cells in the Lymphocytes gate were used for analysis.

ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, left) or ABflo® 610 Rabbit anti-Human CD94 mAb (A26683, 5 μl/Test, right).Cells in the Lymphocytes gate were used for analysis.

Overview

Product nameABflo® 610 Rabbit anti-Human CD94 mAb
Catalog No.A26683
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC60983-ABflo610
Natural killer (NK) cells are a distinct lineage of lymphocytes that mediate cytotoxic activity and secrete cytokines upon immune stimulation. Several genes of the C-type lectin superfamily, including members of the NKG2 family, are expressed by NK cells and may be involved in the regulation of NK cell function. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. Several transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 32-179 of human CD94. (NP_002253.2).
SequenceKNSFTKLSIEPAFTPGPNIELQKDSDCCSCQEKWVGYRCNCYFISSEQKTWNESRHLCASQKSSLLQLQNTDELDFMSSSQQFYWIGLSYSEEHTAWLWENGSALSQYLFPSFETFNTKNCIAYNPNGNALDESCEDKNRYICKQQLI
Gene ID
Swiss Prot
SynonymsCD94
Calculated MW21kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationMembrane, Single-pass type II membrane protein.

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ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)}

Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)

Flow cytometry: 1X10^6 HAP1 cells (negative control, left) and NK-92MI cells (right) were surface-stained with ABflo® 610 Rabbit anti-Human CD94 mAb (A26683, 5 μl/Test, orange line) or ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)}

Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)

Flow cytometry: 1X10^6 NK-92MI cells were surface-stained with ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, left) or ABflo® 610 Rabbit anti-Human CD94 mAb (A26683, 5 μl/Test, right).
ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)}

Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 610 Rabbit anti-Human CD94 mAb (A26683, 5 μl/Test, orange line) or ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).Cells in the Lymphocytes gate were used for analysis.
ABclonal:Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)}

Flow CytoMetry - ABflo® 610 Rabbit anti-Human CD94 mAb (A26683)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 610 Rabbit IgG isotype control (A25826, 5 μl/Test, left) or ABflo® 610 Rabbit anti-Human CD94 mAb (A26683, 5 μl/Test, right).Cells in the Lymphocytes gate were used for analysis.

* For research use only. Not for therapeutic or diagnostic purposes.

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