제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Human TIGIT mAb (A23354)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry:1X10^6 293F cells (negative control, Left) and 293F(Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Human TIGIT mAb(A23354, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 293F(Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human TIGIT mAb(A23354, 5 μl/Test, right).

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit anti-Human TIGIT mAb(A23354, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human TIGIT mAb(A23354, 5 μl/Test, right).

Review

Overview

Product name	ABflo® 647 Rabbit anti-Human TIGIT mAb
Catalog No.	A23354
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC59696-ABf647

Background

This gene encodes a member of the PVR (poliovirus receptor) family of immunoglobin proteins. The product of this gene is expressed on several classes of T cells including follicular B helper T cells (TFH). The protein has been shown to bind PVR with high affinity; this binding is thought to assist interactions between TFH and dendritic cells to regulate T cell dependent B cell responses.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-141 of human TIGIT (NP_776160.2).
Sequence	MMTGTIETTGNISAEKGGSIILQCHLSSTTAQVTQVNWEQQDQLLAICNADLGWHISPSFKDRVAPGPGLGLTLQSLTVNDTGEYFCIYHTYPDGTYTGRIFLEVLESSVAEHGARFQIP
Gene ID
Swiss Prot
Synonyms	VSIG9; VSTM3; WUCAM
Calculated MW	18kDa/26kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell surface, plasma membrane
Customer validation	(Human PBMCs)

Documents

Certificate of Compliance

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Lot number

Research Area

ABflo® 647 Rabbit anti-Human TIGIT mAb images

Flow CytoMetry - ABflo® 647 Rabbit anti-Human TIGIT mAb (A23354)

Flow cytometry:1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human TIGIT mAb(A23354, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.