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ACADS / SCAD Rabbit mAb (A20926)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACADS / SCAD Rabbit mAb (A20926)

Western blot analysis of various lysates using ACADS / SCAD Rabbit mAb (A20926) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ACADS / SCAD Rabbit mAb (A20926)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ACADS / SCAD Rabbit mAb (A20926) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACADS / SCAD Rabbit mAb (A20926)

Immunohistochemistry analysis of paraffin-embedded Human liver using ACADS / SCAD Rabbit mAb (A20926) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACADS / SCAD Rabbit mAb (A20926)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ACADS / SCAD Rabbit mAb (A20926) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameACADS / SCAD Rabbit mAb
Catalog No.A20926
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2901
This gene encodes a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with short-chain acyl-CoA dehydrogenase (SCAD) deficiency. Alternative splicing results in two variants which encode different isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 313-412 of human ACADS / SCAD (P16219).
SequenceKLADMALALESARLLTWRAAMLKDNKKPFIKEAAMAKLAASEAATAISHQAIQILGGMGYVTEMPAERHYRDARITEIYEGTSEIQRLVIAGHLLRSYRS
Gene ID
Swiss Prot
SynonymsSCAD; ACAD3; ACADS / SCAD
Calculated MW44kDa
Observed MW41kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, Jurkat, Mouse liver, Mouse skeletal muscle, Mouse heart, Rat liver, Rat skeletal muscle, Rat heart
Cellular locationMitochondrion matrix.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - ACADS / SCAD Rabbit mAb (A20926)}

Western blot - ACADS / SCAD Rabbit mAb (A20926)

Western blot analysis of various lysates using ACADS / SCAD Rabbit mAb (A20926) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ACADS / SCAD Rabbit mAb (A20926)}

Immunohistochemistry - ACADS / SCAD Rabbit mAb (A20926)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using ACADS / SCAD Rabbit mAb (A20926) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACADS / SCAD Rabbit mAb (A20926)}

Immunohistochemistry - ACADS / SCAD Rabbit mAb (A20926)

Immunohistochemistry analysis of paraffin-embedded Human liver using ACADS / SCAD Rabbit mAb (A20926) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACADS / SCAD Rabbit mAb (A20926)}

Immunohistochemistry - ACADS / SCAD Rabbit mAb (A20926)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using ACADS / SCAD Rabbit mAb (A20926) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)