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ACADVL Rabbit pAb (A7865)

Publication (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACADVL Rabbit pAb (A7865)

Western blot analysis of extracts of various cell lines, using ACADVL antibody (A7865) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - ACADVL Rabbit pAb (A7865)

Immunofluorescence analysis of HeLa cells using ACADVL antibody (A7865) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameACADVL Rabbit pAb
Catalog No.A7865
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is targeted to the inner mitochondrial membrane where it catalyzes the first step of the mitochondrial fatty acid beta-oxidation pathway. This acyl-Coenzyme A dehydrogenase is specific to long-chain and very-long-chain fatty acids. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human ACADVL (NP_000009.1).
SequenceMQAARMAASLGRQLLRLGGGSSRLTALLGQPRPGPARRPYAGGAAQLALDKSDSHPSDALTRKKPAKAESKSFAVGMFKGQLTTDQVFPYPSVLNEEQTQFLKELVEPVSRFFEEVNDPAKNDALEMVEETTWQGLKELG
Gene ID
Swiss Prot
SynonymsACAD6; LCACD; VLCAD
Calculated MW70kDa
Observed MW70kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesK-562, LO2, A-549, U-87MG, HeLa, SW620, Mouse liver, Rat liver
Cellular locationMitochondrion inner membrane
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - ACADVL Rabbit pAb (A7865)}

Western blot - ACADVL Rabbit pAb (A7865)

Western blot analysis of extracts of various cell lines, using ACADVL antibody (A7865) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - ACADVL Rabbit pAb (A7865)}

Immunofluorescence - ACADVL Rabbit pAb (A7865)

Immunofluorescence analysis of HeLa cells using ACADVL antibody (A7865) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7865? Please let us know so that we can cite the reference in this datasheet.