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ACOX1 Rabbit pAb (A8091)

Publications (10) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACOX1 Rabbit pAb (A8091)

Western blot analysis of various lysates using ACOX1 Rabbit pAb (A8091) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ACOX1 Rabbit pAb (A8091)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using ACOX1 Rabbit pAb (A8091) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACOX1 Rabbit pAb (A8091)

Immunohistochemistry analysis of paraffin-embedded Human liver using ACOX1 Rabbit pAb (A8091) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACOX1 Rabbit pAb (A8091)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ACOX1 Rabbit pAb (A8091) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACOX1 Rabbit pAb (A8091)

Immunohistochemistry analysis of paraffin-embedded Rat liver using ACOX1 Rabbit pAb (A8091) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameACOX1 Rabbit pAb
Catalog No.A8091
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human ACOX1 (NP_004026.2).
SequenceMNPDLRRERDSASFNPELLTHILDGSPEKTRRRREIENMILNDPDFQHEDLNFLTRSQRYEVAVRKSAIMVKKMREFGIADPDEIMWFKNFVHRGRPEPLDLHLGMFLPTLLHQATAEQQERFFMPAWNLEIIGTYAQTEMGHGTHLRGLETTATYDPETQEFILNSPTVTSIKWWPGGLGKTSNHAIVLAQLITKGKCYGLHAFIVPIREIGTHKPLPGITVGDIGPKFGYDEIDNGYLKMDNHRIPRENMLMKYAQVKPDGTYVKPLS
Gene ID
Swiss Prot
SynonymsAOX; ACOX; SCOX; MITCH; PALMCOX; ACOX1
Calculated MW74kDa
Observed MW74kDa/50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse liver, Mouse kidney, Mouse heart, Rat liver
Cellular locationPeroxisome.
Customer validation

WB(Homo sapiens, Sus scrofa, Mus musculus, Rattus norvegicus)

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - ACOX1 Rabbit pAb (A8091)}

Western blot - ACOX1 Rabbit pAb (A8091)

Western blot analysis of various lysates using ACOX1 Rabbit pAb (A8091) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ACOX1 Rabbit pAb (A8091)}

Immunohistochemistry - ACOX1 Rabbit pAb (A8091)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using ACOX1 Rabbit pAb (A8091) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACOX1 Rabbit pAb (A8091)}

Immunohistochemistry - ACOX1 Rabbit pAb (A8091)

Immunohistochemistry analysis of paraffin-embedded Human liver using ACOX1 Rabbit pAb (A8091) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACOX1 Rabbit pAb (A8091)}

Immunohistochemistry - ACOX1 Rabbit pAb (A8091)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ACOX1 Rabbit pAb (A8091) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACOX1 Rabbit pAb (A8091)}

Immunohistochemistry - ACOX1 Rabbit pAb (A8091)

Immunohistochemistry analysis of paraffin-embedded Rat liver using ACOX1 Rabbit pAb (A8091) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A8091? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)