제품 > 항체 > 다중클론항체(pAb)

ACTR6 Rabbit pAb (A13227)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACTR6 Rabbit pAb (A13227)

Western blot analysis of various lysates using ACTR6 Rabbit pAb (A13227) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameACTR6 Rabbit pAb
Catalog No.A13227
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable nucleosome binding activity. Predicted to be involved in histone exchange. Predicted to be located in nucleus. Predicted to be part of Swr1 complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 237-396 of human ACTR6 (NP_071941.1).
SequenceVLPDFSTIKKGFCKPREEMVLSGKYKSGEQILRLANERFAVPEILFNPSDIGIQEMGIPEAIVYSIQNLPEEMQPHFFKNIVLTGGNSLFPGFRDRVYSEVRCLTPTDYDVSVVLPENPITYAWEGGKLISENDDFEDMVVTREDYEENGHSVCEEKFDI
Gene ID
Swiss Prot
SynonymsARP6; CDA12; hARP6; hARPX; HSPC281; MSTP136; ACTR6
Calculated MW46kDa
Observed MW46kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesJurkat, NIH/3T3, Mouse thymus, Rat thymus
Cellular locationCytoplasm, Nucleus, cytoskeleton
Customer validation

WB(Homo sapiens)

    ABclonal:Western blot - ACTR6 Rabbit pAb (A13227)}

    Western blot - ACTR6 Rabbit pAb (A13227)

    Western blot analysis of various lysates using ACTR6 Rabbit pAb (A13227) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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