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ALK Rabbit pAb (A0766)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ALK Rabbit pAb (A0766)

Western blot analysis of lysates from SH-SY5Y cells, using ALK Rabbit pAb (A0766) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - ALK Rabbit pAb (A0766)

Immunofluorescence analysis of SH-SY5Y cells using ALK Rabbit pAb (A0766) at dilution of 1:200. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ALK Rabbit pAb (A0766)

Immunofluorescence analysis of Neuro-2a cells using ALK Rabbit pAb (A0766) at dilution of 1:200. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameALK Rabbit pAb
Catalog No.A0766
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1521-1620 of human ALK (NP_004295.2).
SequencePIAKKEPHDRGNLGLEGSCTVPPNVATGRLPGASLLLEPSSLTANMKEVPLFRLRHFPCGNVNYGYQQQGLPLEAATAPGAGHYEDTILKSKNSMNQPGP
Gene ID
Swiss Prot
SynonymsALK1; CD246; NBLST3; ALK
Calculated MW176kDa
Observed MW140kDa/220kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesPC-3, Mouse testis
Cellular locationCell membrane, Single-pass type I membrane protein.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - ALK Rabbit pAb (A0766)}

Western blot - ALK Rabbit pAb (A0766)

Western blot analysis of lysates from SH-SY5Y cells, using ALK Rabbit pAb (A0766) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - ALK Rabbit pAb (A0766)}

Immunofluorescence - ALK Rabbit pAb (A0766)

Immunofluorescence analysis of SH-SY5Y cells using ALK Rabbit pAb (A0766) at dilution of 1:200. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ALK Rabbit pAb (A0766)}

Immunofluorescence - ALK Rabbit pAb (A0766)

Immunofluorescence analysis of Neuro-2a cells using ALK Rabbit pAb (A0766) at dilution of 1:200. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (2)

Secondary Antibodies (26)