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ALOX5 Rabbit pAb (A2158)

Publications (5) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ALOX5 Rabbit pAb (A2158)

Western blot analysis of lysates from Mouse lung, using ALOX5 Rabbit pAb (A2158) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - ALOX5 Rabbit pAb (A2158)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using ALOX5 Rabbit pAb (A2158) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - ALOX5 Rabbit pAb (A2158)

Immunofluorescence analysis of C6 cells using ALOX5 Rabbit pAb (A2158) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameALOX5 Rabbit pAb
Catalog No.A2158
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8, 11, 14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7, 9-trans-11, 14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of ALOX5 (NP_000689.1).
SequenceMPSYTVTVATGSQWFAGTDDYIYLSLVGSAGCSEKHLLDKPFYNDFERGAVDSYDVTVDEELGEIQLVRIEKRKYWLNDDWYLKYITLKTPHGDYIEFPCYRWITGDVEVVLRDGRAKLARDDQIHILKQHRRKELETRQKQYRWMEWNPGFPLSIDAKCHKDLPRDIQFDSEKGVDFVLNYSKAMENLFINRFMHMFQS
Gene ID
Swiss Prot
Synonyms5-LO; 5LPG; LOG5; 5-LOX; ALOX5
Calculated MW78kDa
Observed MW75kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse lung
Cellular locationCytoplasm, Nucleus matrix, Nucleus membrane, Peripheral membrane protein.
Customer validation

WB(Ovis aries, Homo sapiens)

IF(Other)

IHC(Other)

WB(Other)

IHC(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - ALOX5 Rabbit pAb (A2158)}

Western blot - ALOX5 Rabbit pAb (A2158)

Western blot analysis of lysates from Mouse lung, using ALOX5 Rabbit pAb (A2158) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - ALOX5 Rabbit pAb (A2158)}

Immunohistochemistry - ALOX5 Rabbit pAb (A2158)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using ALOX5 Rabbit pAb (A2158) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - ALOX5 Rabbit pAb (A2158)}

Immunofluorescence - ALOX5 Rabbit pAb (A2158)

Immunofluorescence analysis of C6 cells using ALOX5 Rabbit pAb (A2158) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (3)

Secondary Antibodies (26)