제품 > 항체 > 다중클론항체(pAb)

ANTXR2 Rabbit pAb (A6526)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ANTXR2 Rabbit pAb (A6526)

Western blot analysis of various lysates using ANTXR2 Rabbit pAb (A6526) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - ANTXR2 Rabbit pAb (A6526)

Immunohistochemistry analysis of paraffin-embedded Human stomach using ANTXR2 Rabbit pAb (A6526) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ANTXR2 Rabbit pAb (A6526)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ANTXR2 Rabbit pAb (A6526) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameANTXR2 Rabbit pAb
Catalog No.A6526
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a receptor for anthrax toxin. The protein binds to collagen IV and laminin, suggesting that it may be involved in extracellular matrix adhesion. Mutations in this gene cause juvenile hyaline fibromatosis and infantile systemic hyalinosis. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 34-300 of human ANTXR2 (NP_001139266.1).
SequenceQEQPSCRRAFDLYFVLDKSGSVANNWIEIYNFVQQLAERFVSPEMRLSFIVFSSQATIILPLTGDRGKISKGLEDLKRVSPVGETYIHEGLKLANEQIQKAGGLKTSSIIIALTDGKLDGLVPSYAEKEAKISRSLGASVYCVGVLDFEQAQLERIADSKEQVFPVKGGFQALKGIINSILAQSCTEILELQPSSVCVGEEFQIVLSGRGFMLGSRNGSVLCTYTVNETYTTSVKPVSVQLNSMLCPAPILNKAGETLDVSVSFNGG
Gene ID
Swiss Prot
SynonymsHFS; ISH; JHF; CMG2; CMG-2; ANTXR2
Calculated MW54kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse eye, Rat lung
Cellular locationCell membrane, Endoplasmic reticulum membrane, Secreted, Single-pass type I membrane protein, Single-pass type I membrane protein.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - ANTXR2 Rabbit pAb (A6526)}

    Western blot - ANTXR2 Rabbit pAb (A6526)

    Western blot analysis of various lysates using ANTXR2 Rabbit pAb (A6526) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunohistochemistry - ANTXR2 Rabbit pAb (A6526)}

    Immunohistochemistry - ANTXR2 Rabbit pAb (A6526)

    Immunohistochemistry analysis of paraffin-embedded Human stomach using ANTXR2 Rabbit pAb (A6526) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
    ABclonal:Immunohistochemistry - ANTXR2 Rabbit pAb (A6526)}

    Immunohistochemistry - ANTXR2 Rabbit pAb (A6526)

    Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ANTXR2 Rabbit pAb (A6526) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A6526? Please let us know so that we can cite the reference in this datasheet.