제품 > 항체 > 단일클론항체(mAb)

APC Rabbit anti-Human CD275/B7-H2/ICOSL mAb (A26268)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 SH-SY5Y cells (negative control, left) and Daudi cells (right) were surface-stained with APC Rabbit anti-Human CD275/B7-H2/ICOSL mAb (A26268, 5 μl/Test, orange line) or APC Rabbit IgG isotype control (A24173, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Daudi cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD275/B7-H2/ICOSL mAb (A26268, 5 μl/Test, right).

Overview

Product name	APC Rabbit anti-Human CD275/B7-H2/ICOSL mAb
Catalog No.	A26268
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC58551-APC

Background

Enables identical protein binding activity. Predicted to be involved in T cell receptor signaling pathway and positive regulation of interleukin-4 production. Located in cytoplasmic ribonucleoprotein granule and plasma membrane.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 19-256 of human CD275/B7-H2/ICOSL (NP_056074.1).
Sequence	DTQEKEVRAMVGSDVELSCACPEGSRFDLNDVYVYWQTSESKTVVTYHIPQNSSLENVDSRYRNRALMSPAGMLRGDFSLRLFNVTPQDEQKFHCLVLSQSLGFQEVLSVEVTLHVAANFSVPVVSAPHSPSQDELTFTCTSINGYPRPNVYWINKTDNSLLDQALQNDTVFLNMRGLYDVVSVLRIARTPSVNIGCCIENVLLQQNLTVGSQTGNDIGERDKITENPVSTGEKNAAT
Gene ID
Swiss Prot
Synonyms	B7h; B7H2; GL50; B7-H2; B7RP1; CD275; ICOSL; LICOS; B7RP-1; ICOS-L
Calculated MW	20kDa/33kDa/34kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane , Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Research Area

APC Rabbit anti-Human CD275/B7-H2/ICOSL mAb images

Flow CytoMetry - APC Rabbit anti-Human CD275/B7-H2/ICOSL mAb (A26268)

Flow cytometry: 1X10^6 Daudi cells were surface-stained with APC Rabbit IgG isotype control (A24173, 5 μl/Test, left) or APC Rabbit anti-Human CD275/B7-H2/ICOSL mAb (A26268, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.