제품 > 항체 > 다중클론항체(pAb)

ATF3 Rabbit pAb (A13469)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from 293T cells, using ATF3 Rabbit pAb (A13469) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from RAW264.7 cells, using ATF3 Rabbit pAb (A13469) at 1:1000 dilution.Raw264.7 cells were treated by LPS (1 μg/ml) at 37℃ for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ATF3 Rabbit pAb (A13469)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using ATF3 Rabbit pAb (A13469) at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product name	ATF3 Rabbit pAb
Catalog No.	A13469
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-181 of human ATF3 (NP_001665.1).
Sequence	MMLQHPGQVSASEVSASAIVPCLSPPGSLVFEDFANLTPFVKEELRFAIQNKHLCHRMSSALESVTVSDRPLGVSITKAEVAPEEDERKKRRRERNKIAAAKCRNKKKEKTECLQKESEKLESVNAELKAQIEELKNEKQHLIYMLNLHRPTCIVRAQNGRTPEDERNLFIQQIKEGTLQS
Gene ID
Swiss Prot
Synonyms	ATF3
Calculated MW	21kDa
Observed MW	23kDa/24kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	293T, RAW264.7 treated by LPS
Cellular location	Nucleus
Customer validation	FC(Homo sapiens) WB(Homo sapiens, Mus musculus, Anatinae) IF(Rattus norvegicus, Homo sapiens) IHC(Homo sapiens) IF(Mus musculus)

Documents

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Research Area

ATF3 Rabbit pAb images

Western blot - ATF3 Rabbit pAb (A13469)

Immunohistochemistry - ATF3 Rabbit pAb (A13469)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for ATF3 Rabbit pAb (A13469)

Product:

ATF3 Rabbit pAb

Journal:

iScience

Application:

IF:

5.8

Species:

Homo sapiens

PMID:

35996586

Title:

Transcriptional responses to injury of regenerative lung alveolar epithelium - PMC

References for ATF3 Rabbit pAb (A13469)

Product:

ATF3 Rabbit pAb

Journal:

Human gene therapy

Application:

IF:

3.9

Species:

Homo sapiens

PMID:

36373826

Title:

HIV Tat-conjugated Histone H3 peptides induce tumor cell death via cellular stress responses

References for ATF3 Rabbit pAb (A13469)

Product:

ATF3 Rabbit pAb

Journal:

Redox biology

Application:

IF:

10.7

Species:

Rattus norvegicus

PMID:

37931471

Title:

Dimethyl fumarate ameliorates erectile dysfunction in bilateral cavernous nerve injury rats by inhibiting oxidative stress and NLRP3 inflammasome-mediated

References for ATF3 Rabbit pAb (A13469)

Product:

ATF3 Rabbit pAb

Journal:

Tissue Cell

Application:

IHC

IF:

2.7

Species:

Homo sapiens

PMID:

34666282

Title:

ATF3 regulates oxidative stress and extracellular matrix degradation via p38/Nrf2 signaling pathway in pelvic organ prolapse

References for ATF3 Rabbit pAb (A13469)

Product:

ATF3 Rabbit pAb

Journal:

Cell Discovery

Application:

IF:

33.5

Species:

Homo sapiens

PMID:

37816761

Title:

Self-recruited neutrophils trigger over-activated innate immune response and phenotypic change of cardiomyocytes in fulminant viral myocarditis

References for ATF3 Rabbit pAb (A13469)

Product:

ATF3 Rabbit pAb

Journal:

Cellular oncology (Dordrecht)

Application:

IF:

4.9

Species:

Mus musculus

PMID:

38097870

Title:

ATF3-induced activation of the NF-B pathway results in acquired PARP inhibitor resistance in pancreatic adenocarcinoma

References for ATF3 Rabbit pAb (A13469)

Product:

ATF3 Rabbit pAb

Journal:

Neuroendocrinology

Application:

WB, IF

IF:

3.2

Species:

Mus musculus

PMID:

34763342

Title:

Transcriptomic Analysis Reveals that Activating Transcription Factor 3/c-Jun/Lgals3 Axis Is Associated with Central Diabetes Insipidus after Hypothalamic Injury

References for ATF3 Rabbit pAb (A13469)

Product:

ATF3 Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

5.6

Species:

Mus musculus

PMID:

36675183