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ATF5 Rabbit pAb (A18155)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ATF5 Rabbit pAb (A18155)

Western blot analysis of lysates from rat lung, using ATF5 Rabbit pAb (A18155) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ATF5 Rabbit pAb (A18155)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using ATF5 Rabbit pAb (A18155) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ATF5 Rabbit pAb (A18155)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using ATF5 Rabbit pAb (A18155) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - ATF5 Rabbit pAb (A18155)

Immunofluorescence analysis of PC-12 cells using ATF5 Rabbit pAb (A18155) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ATF5 Rabbit pAb (A18155)

Immunofluorescence analysis of U2OS cells using ATF5 Rabbit pAb (A18155) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameATF5 Rabbit pAb
Catalog No.A18155
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables several functions, including DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II transcription regulatory region sequence-specific DNA binding activity; and tubulin binding activity. Involved in several processes, including fat cell differentiation; regulation of cell cycle process; and regulation of transcription, DNA-templated. Located in centrosome; cytosol; and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 101-282 of human ATF5 (NP_036200.2).
SequenceMASLLKKELEQMEDFFLDAPPLPPPSPPPLPPPPLPPAPSLPLSLPSFDLPQPPVLDTLDLLAIYCRNEAGQEEVGMPPLPPPQQPPPPSPPQPSRLAPYPHPATTRGDRKQKKRDQNKSAALRYRQRKRAEGEALEGECQGLEARNRELKERAESVEREIQYVKDLLIEVYKARSQRTRSC
Gene ID
Swiss Prot
SynonymsATFX; HMFN0395; ATF5
Calculated MW31kDa
Observed MW31kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesrat lung
Cellular locationcentrosome, cytosol, nucleoplasm, nucleus.
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - ATF5 Rabbit pAb (A18155)}

Western blot - ATF5 Rabbit pAb (A18155)

Western blot analysis of lysates from rat lung, using ATF5 Rabbit pAb (A18155) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ATF5 Rabbit pAb (A18155)}

Immunohistochemistry - ATF5 Rabbit pAb (A18155)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using ATF5 Rabbit pAb (A18155) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ATF5 Rabbit pAb (A18155)}

Immunohistochemistry - ATF5 Rabbit pAb (A18155)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using ATF5 Rabbit pAb (A18155) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - ATF5 Rabbit pAb (A18155)}

Immunofluorescence - ATF5 Rabbit pAb (A18155)

Immunofluorescence analysis of PC-12 cells using ATF5 Rabbit pAb (A18155) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ATF5 Rabbit pAb (A18155)}

Immunofluorescence - ATF5 Rabbit pAb (A18155)

Immunofluorescence analysis of U2OS cells using ATF5 Rabbit pAb (A18155) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (1)

Secondary Antibodies (26)