Review (1)Publications (6) Datasheet
Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using ATGL/PNPLA2 Rabbit mAb (A5126) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
| Product name | ATGL/PNPLA2 Rabbit mAb |
|---|---|
| Catalog No. | A5126 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC1192 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 300-394 of human ATGL / PNPLA2 (NP_065109.1). |
|---|---|
| Sequence | LPARLNEALLEACVEPTDLLTTLSNMLPVRLATAMMVPYTLPLESALSFTIRLLEWLPDVPEDIRWMKEQTGSICQYLVMRAKRKLGRHLPSRLP |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik; ATGL/PNPLA2 |
| Calculated MW | 55kDa |
| Observed MW | 55kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | HeLa, A-431, U-87MG, Mouse brain, Rat brain, Mouse kidney, Rat kidney, Mouse heart, Rat heart |
| Cellular location | Cell membrane, Lipid droplet, Single-pass type II membrane protein. |
| Customer validation | (Jurkat、RPMI-8226、PC-3、HepG2、HeLa、A549、T47D) WB(Mus musculus, Homo sapiens) IHC(Mus musculus) |
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