Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using AXIN2 Rabbit pAb (A2513) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): A-431.
Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded rat brain using AXIN2 Rabbit pAb (A2513) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using AXIN2 Rabbit pAb (A2513) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
| Product name | AXIN2 Rabbit pAb |
|---|---|
| Catalog No. | A2513 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 743-843 of human AXIN2 (NP_004646.3). |
|---|---|
| Sequence | APEDHKEPKKLAGVHALQASELVVTYFFCGEEIPYRRMLKAQSLTLGHFKEQLSKKGNYRYYFKKASDEFACGAVFEEIWEDETVLPMYEGRILGKVERID |
| Gene ID | |
| Swiss Prot | |
| Synonyms | AXIL; ODCRCS; AXIN2 |
| Calculated MW | 94kDa |
| Observed MW | 98kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | A549, Hep G2, Mouse placenta |
| Cellular location | Cytoplasm |
| Customer validation | WB(Homo sapiens, Mus musculus) IHC(Mus musculus, Rattus norvegicus) IF(Homo sapiens) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A2513? Please let us know so that we can cite the reference in this datasheet.
