Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using BACE1 Rabbit mAb (A11533) at 1：1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Western blot analysis of lysates from SH-SY5Y cells, using BACE1 Rabbit mAb (A11533) at 1：1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.
| Product name | BACE1 Rabbit mAb |
|---|---|
| Catalog No. | A11533 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| CloneNo. | ARC0696 |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 402-501 of human BACE1 (P56817). |
|---|---|
| Sequence | FYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK |
| Gene ID | |
| Swiss Prot | |
| Synonyms | ASP2; BACE; HSPC104; BACE1 |
| Calculated MW | 56kDa |
| Observed MW | 70kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Key application | Western blotting |
| Positive samples | SH-SY5Y, Mouse brain, Rat brain |
| Cellular location | Cell surface, Cytoplasmic vesicle membrane, Endoplasmic reticulum, Endosome, Golgi apparatus, Membrane, Single-pass type I membrane protein, trans-Golgi network. |
| Customer validation | WB(Homo sapiens, Mus musculus, Rattus norvegicus) |
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* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A11533? Please let us know so that we can cite the reference in this datasheet.
