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BANF1 Rabbit mAb (A3726)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BANF1 Rabbit mAb (A3726)

Western blot analysis of various lysates using BANF1 Rabbit mAb (A3726) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - BANF1 Rabbit mAb (A3726)

Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using BANF1 Rabbit mAb (A3726) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - BANF1 Rabbit mAb (A3726)

Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using BANF1 Rabbit mAb (A3726) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - BANF1 Rabbit mAb (A3726)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using BANF1 Rabbit mAb (A3726) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - BANF1 Rabbit mAb (A3726)

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using BANF1 Rabbit mAb (A3726) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - BANF1 Rabbit mAb (A3726)

Immunofluorescence analysis of HeLa cells using BANF1 Rabbit mAb (A3726) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameBANF1 Rabbit mAb
Catalog No.A3726
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2085
The protein encoded by this gene was first identified by its ability to protect retroviruses from intramolecular integration and therefore promote intermolecular integration into the host cell genome. The protein forms a homodimer which localizes to both the nucleus and cytoplasm and is specifically associated with chromosomes during mitosis. This protein binds to double stranded DNA in a non-specific manner and also binds to LEM-domain containing proteins of the nuclear envelope. This protein is thought to facilitate nuclear reassembly by binding with both DNA and inner nuclear membrane proteins and thereby recruit chromatin to the nuclear periphery. Alternative splicing results in multiple transcript variants encoding the same protein.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-89 of human BANF1 (O75531).
SequenceMTTSQKHRDFVAEPMGEKPVGSLAGIGEVLGKKLEERGFDKAYVVLGQFLVLKKDEDLFREWLKDTCGANAKQSRDCFGCLREWCDAFL
Gene ID
Swiss Prot
SynonymsBAF; NGPS; BCRP1; D14S1460; BANF1
Calculated MW10kDa
Observed MW10kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:200 - 1:800
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, HepG2
Cellular locationNucleus, Cytoplasm, Chromosome.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - BANF1 Rabbit mAb (A3726)}

    Western blot - BANF1 Rabbit mAb (A3726)

    Western blot analysis of various lysates using BANF1 Rabbit mAb (A3726) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Immunohistochemistry - BANF1 Rabbit mAb (A3726)}

    Immunohistochemistry - BANF1 Rabbit mAb (A3726)

    Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using BANF1 Rabbit mAb (A3726) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - BANF1 Rabbit mAb (A3726)}

    Immunohistochemistry - BANF1 Rabbit mAb (A3726)

    Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using BANF1 Rabbit mAb (A3726) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - BANF1 Rabbit mAb (A3726)}

    Immunohistochemistry - BANF1 Rabbit mAb (A3726)

    Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using BANF1 Rabbit mAb (A3726) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - BANF1 Rabbit mAb (A3726)}

    Immunohistochemistry - BANF1 Rabbit mAb (A3726)

    Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using BANF1 Rabbit mAb (A3726) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
    ABclonal:Immunofluorescence - BANF1 Rabbit mAb (A3726)}

    Immunofluorescence - BANF1 Rabbit mAb (A3726)

    Immunofluorescence analysis of HeLa cells using BANF1 Rabbit mAb (A3726) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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