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BOK Rabbit pAb (A16354)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - BOK Rabbit pAb (A16354)

Western blot analysis of various lysates using BOK Rabbit pAb (A16354) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Western blot - BOK Rabbit pAb (A16354)

Western blot analysis of various lysates using BOK Rabbit pAb (A16354)at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): U-937
Exposure time: 90s.

ABclonal:Immunohistochemistry - BOK Rabbit pAb (A16354)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using BOK Rabbit pAb (A16354) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - BOK Rabbit pAb (A16354)

Immunohistochemistry analysis of paraffin-embedded Rat lung using BOK Rabbit pAb (A16354) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - BOK Rabbit pAb (A16354)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using BOK Rabbit pAb (A16354) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - BOK Rabbit pAb (A16354)

Immunofluorescence analysis of MCF7 cells using BOK Rabbit pAb (A16354) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - BOK Rabbit pAb (A16354)

Immunofluorescence analysis of NIH/3T3 cells using BOK Rabbit pAb (A16354) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameBOK Rabbit pAb
Catalog No.A16354
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the BCL2 family, members of which form homo- or heterodimers, and act as anti- or proapoptotic regulators that are involved in a wide variety of cellular processes. Studies in rat show that this protein has restricted expression in reproductive tissues, interacts strongly with some antiapoptotic BCL2 proteins, not at all with proapoptotic BCL2 proteins, and induces apoptosis in transfected cells. Thus, this protein represents a proapoptotic member of the BCL2 family.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human BOK (NP_115904.1).
SequenceMEVLRRSSVFAAEIMDAFDRSPTDKELVAQAKALGREYVHARLLRAGLSWSAPERAAPVPGRLAEVCAVLLRLGDELEMIRPSVYRNVARQLHISLQSEP
Gene ID
Swiss Prot
SynonymsBOKL; BCL2L9; BOK
Calculated MW23kDa
Observed MW22kDa/23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat testis, MCF7, Mouse spleen, Rat ovary
Cellular locationEndoplasmic reticulum membrane, Golgi apparatus membrane, Single-pass membrane protein.

Documents

Certificate of Compliance

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ABclonal:Western blot - BOK Rabbit pAb (A16354)}

Western blot - BOK Rabbit pAb (A16354)

Western blot analysis of various lysates using BOK Rabbit pAb (A16354) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Western blot - BOK Rabbit pAb (A16354)}

Western blot - BOK Rabbit pAb (A16354)

Western blot analysis of various lysates using BOK Rabbit pAb (A16354)at 1:10000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): U-937
Exposure time: 90s.
ABclonal:Immunohistochemistry - BOK Rabbit pAb (A16354)}

Immunohistochemistry - BOK Rabbit pAb (A16354)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using BOK Rabbit pAb (A16354) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - BOK Rabbit pAb (A16354)}

Immunohistochemistry - BOK Rabbit pAb (A16354)

Immunohistochemistry analysis of paraffin-embedded Rat lung using BOK Rabbit pAb (A16354) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - BOK Rabbit pAb (A16354)}

Immunohistochemistry - BOK Rabbit pAb (A16354)

Immunohistochemistry analysis of paraffin-embedded Rat ovary using BOK Rabbit pAb (A16354) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - BOK Rabbit pAb (A16354)}

Immunofluorescence - BOK Rabbit pAb (A16354)

Immunofluorescence analysis of MCF7 cells using BOK Rabbit pAb (A16354) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - BOK Rabbit pAb (A16354)}

Immunofluorescence - BOK Rabbit pAb (A16354)

Immunofluorescence analysis of NIH/3T3 cells using BOK Rabbit pAb (A16354) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)