제품 > 항체 > 다중클론항체(pAb)

Bcl9 Rabbit pAb (A17209)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Immunofluorescence analysis of U2OS cells using Bcl9 Rabbit pAb (A17209) at dilution of 100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Bcl9 Rabbit pAb
Catalog No.	A17209
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Enables beta-catenin binding activity. Acts upstream of or within several processes, including myotube differentiation involved in skeletal muscle regeneration; skeletal muscle cell differentiation; and somatic stem cell population maintenance. Located in cis-Golgi network and nucleus. Is expressed in several structures, including brain; eye; head surface ectoderm; metanephros; and thymus primordium. Orthologous to human BCL9 (BCL9 transcription coactivator).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-500 of mouse Bcl9. (Q9D219).
Sequence	MHPSNPKVRSSPSGNTQSSPKSKQEVMVRPPTVMSPSGNPQLDSKFSNQGKPGGSASQSQPSPCDSKSGGHTPKALPGPGGSMGLKNGAGNGAKGKGKRERSISADSFDQRDPGTPNDDSDIKECNSADHIKSQESQHTPHSMTPSTATAPRSSTPSHGQTPAPEPISAQKTPAKVVYVFSTEMANKAAEAVLKGQVETIVSFHIQNISNSKSERSTAPLNTQIPTLRNDPKPLPQQPPAPANQDQNSSQNARLQPTPPIQAPAPKPTAAPRPLDRESPGVENKLIPPVGSPGSSTPLPPDGTGPNSTPNNRAVTPVSQGSNSSSADPKAPPPPPVSGGEPPTLGENPDGLSQEQLEHRERSLQTLRDIQRMLFPDEKEFTAGQTGGPQQNTGVLDGPQKKPDGPIQAMMSQSQSLGKGPGPRTDVGAPFGPQGHRDVPFSPDEMVPPNMSSQSGPIGPDHLDHMTPEQIAWLKLQQEFYEEKRRKQEQVVVQQCSLQDM
Gene ID
Swiss Prot
Synonyms	Gm130; 2610202E01Rik; 8030475K17Rik; A330041G23Rik; Bcl9
Calculated MW	149kDa
Observed MW	Refer to figures

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples
Cellular location	Nucleus