제품 > 항체 > 다중클론항체(pAb)

Btbd11 Rabbit pAb (A20192)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Btbd11 Rabbit pAb (A20192)

Western blot analysis of lysates from Mouse brain, using Btbd11 Rabbit pAb (A20192) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Overview

Product nameBtbd11 Rabbit pAb
Catalog No.A20192
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to enable protein heterodimerization activity. Acts upstream of or within SMAD protein signal transduction. Predicted to be located in membrane. Predicted to be integral component of membrane. Is expressed in central nervous system; dorsal root ganglion; genitourinary system; and thymus primordium. Orthologous to human BTBD11 (BTB domain containing 11).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-120 of mouse Btbd11 (NP_082985.2).
SequenceMARRGKKPVVRTLEDLTLDSGYGGAADSVRSSNLSLCCSDSHPASPYGGSCWPPLADSMHSRHNSFDTVNTALVEDSEGLDCAGQHCSRLLPDLDEVPWTLQELELLLLRSRDPRAGPAV
Gene ID
Swiss Prot
SynonymsBtbd11; 6330404E16Rik
Calculated MW122kDa
Observed MW121kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesMouse brain
Cellular location
Customer validation

WB(Mus musculus)

IHC(Mus musculus)

    ABclonal:Western blot - Btbd11 Rabbit pAb (A20192)}

    Western blot - Btbd11 Rabbit pAb (A20192)

    Western blot analysis of lysates from Mouse brain, using Btbd11 Rabbit pAb (A20192) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A20192? Please let us know so that we can cite the reference in this datasheet.