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CBL Rabbit pAb (A7881)

Publications (3) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CBL Rabbit pAb (A7881)

Western blot analysis of various lysates using CBL Rabbit pAb (A7881) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.

ABclonal:Immunohistochemistry - CBL Rabbit pAb (A7881)

Immunohistochemistry analysis of paraffin-embedded Mouse stomach using CBL Rabbit pAb (A7881) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CBL Rabbit pAb (A7881)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using CBL Rabbit pAb (A7881) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - CBL Rabbit pAb (A7881)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using CBL Rabbit pAb (A7881) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameCBL Rabbit pAb
Catalog No.A7881
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a proto-oncogene that encodes a RING finger E3 ubiquitin ligase. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes (E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia, and expansion of CGG repeats in the 5' UTR has been associated with Jacobsen syndrome. Mutations in this gene are also the cause of Noonan syndrome-like disorder.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 850-906 of human CBL (NP_005179.2).
SequenceAATASPQLSSEIENLMSQGYSYQDIQKALVIAQNNIEMAKNILREFVSISSPAHVAT
Gene ID
Swiss Prot
SynonymsCBL2; NSLL; C-CBL; RNF55; FRA11B; CBL
Calculated MW100kDa
Observed MW120kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesJurkat, Raji, Mouse thymus
Cellular locationCell membrane, Cytoplasm
Customer validation

WB(Homo sapiens, Rattus norvegicus)

IHC(Homo sapiens, Rattus norvegicus)

IF(Homo sapiens, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CBL Rabbit pAb (A7881)}

Western blot - CBL Rabbit pAb (A7881)

Western blot analysis of various lysates using CBL Rabbit pAb (A7881) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s.
ABclonal:Immunohistochemistry - CBL Rabbit pAb (A7881)}

Immunohistochemistry - CBL Rabbit pAb (A7881)

Immunohistochemistry analysis of paraffin-embedded Mouse stomach using CBL Rabbit pAb (A7881) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CBL Rabbit pAb (A7881)}

Immunohistochemistry - CBL Rabbit pAb (A7881)

Immunohistochemistry analysis of paraffin-embedded Rat kidney using CBL Rabbit pAb (A7881) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - CBL Rabbit pAb (A7881)}

Immunohistochemistry - CBL Rabbit pAb (A7881)

Immunohistochemistry analysis of paraffin-embedded Mouse liver using CBL Rabbit pAb (A7881) (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7881? Please let us know so that we can cite the reference in this datasheet.

항체 (4)

Secondary Antibodies (25)