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CD11a/LFA-1A/ITGAL Rabbit pAb (A1644)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using CD11a/LFA-1A/ITGAL Rabbit pAb (A1644) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CD11a/LFA-1A/ITGAL Rabbit pAb (A1644)

Immunohistochemistry analysis of paraffin-embedded rat spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (A1644) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded human appendix using CD11a/LFA-1A/ITGAL Rabbit pAb (A1644) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry analysis of paraffin-embedded mouse spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (A1644) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - CD11a/LFA-1A/ITGAL Rabbit pAb (A1644)

Immunofluorescence analysis of paraffin-embedded rat spleen using CD11a/LFA-1A/ITGAL Rabbit pAb (A1644) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of THP-1 cells using CD11a/LFA-1A/ITGAL Rabbit pAb (A1644) at dilution of 1:200. Blue: DAPI for nuclear staining.

Overview

Product name	CD11a/LFA-1A/ITGAL Rabbit pAb
Catalog No.	A1644
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 26-340 of human CD11a/LFA-1A/ITGAL (NP_001107852.1).
Sequence	YNLDVRGARSFSPPRAGRHFGYRVLQVGNGVIVGAPGEGNSTGSLYQCQSGTGHCLPVTLRGSNYTSKYLGMTLATDPTDGSILFAAVQFSTSYKTEFDFSDYVKRKDPDALLKHVKHMLLLTNTFGAINYVATEVFREELGARPDATKVLIIITDGEATDSGNIDAAKDIIRYIIGIGKHFQTKESQETLHKFASKPASEFVKILDTFEKLKDLFTELQKKIYVIEGTSKQDLTSFNMELSSSGISADLSRGHAVVGAVGAKDWAGGFLDLKADLQDDTFIGNEPLTPEVRAGYLGYTVTWLPSRQKTSLLASG
Gene ID
Swiss Prot
Synonyms	CD11A; LFA-1; LFA1A; CD11a/LFA-1A/ITGAL
Calculated MW	129kDa
Observed MW	180kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IHC-P 1:50 - 1:100 IF/ICC 1:50 - 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Molt-4, Mouse spleen, Rat spleen
Cellular location	Membrane, Single-pass type I membrane protein
Customer validation	WB(Homo sapiens) IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

CD11a/LFA-1A/ITGAL Rabbit pAb images

Western blot - CD11a/LFA-1A/ITGAL Rabbit pAb (A1644)

Immunohistochemistry - CD11a/LFA-1A/ITGAL Rabbit pAb (A1644)

Immunofluorescence - CD11a/LFA-1A/ITGAL Rabbit pAb (A1644)

Confocal immunofluorescence analysis of THP-1 cells using CD11a/LFA-1A/ITGAL Rabbit pAb (A1644) at dilution of 1:200. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.