제품 > 항체 > 단일클론항체(mAb)

CD138 Rabbit mAb (A25635)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Immunohistochemistry analysis of CD138 in paraffin-embedded Human tonsil tissue using CD138 Rabbit mAb (A25635) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of CD138 in paraffin-embedded Human small intestine tissue using CD138 Rabbit mAb (A25635) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of CD138 in paraffin-embedded Human liver cancer tissue using CD138 Rabbit mAb (A25635) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.

ABclonal:Flow CytoMetry - CD138 Rabbit mAb (A25635)

Flow cytometry: 1X10^6 HEL cells (negative control, left) and U266 cells (right) were surface-stained with CD138 Rabbit mAb (A25635, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by PE Donkey anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 U266 cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or CD138 Rabbit mAb (A25635, 2 μg/mL, right), followed by PE Donkey anti-Rabbit pAb staining.

Overview

Product name	CD138 Rabbit mAb
Catalog No.	A25635
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC68246

Background

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 23-251 of human CD138 (NP_002988.4).
Sequence	QIVATNLPPEDQDGSGDDSDNFSGSGAGALQDITLSQQTPSTWKDTQLLTAIPTSPEPTGLEATAASTSTLPAGEGPKEGEAVVLPEVEPGLTAREQEATPRPRETTQLPTTHLASTTTATTAQEPATSHPHRDMQPGHHETSTPAGPSQADLHTPHTEDGGPSATERAAEDGASSQLPAAEGSGEQDFTFETSGENTAVVAVEPDRRNQSPVDQGATGASQGLLDRKE
Gene ID
Swiss Prot
Synonyms	SDC; CD138; SYND1; syndecan
Calculated MW	32kDa
Observed MW	Refer to figures

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:1000 - 1:5000 FC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Immunohistochemistry Flow Cytometry
Positive samples
Cellular location	Membrane, Secreted, Single-pass type I membrane protein