제품 > 항체 > 단일클론항체(mAb)

CD146 Rabbit mAb (A24343)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using CD146 Rabbit mAb (A24343) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - CD146 Rabbit mAb (A24343)

Immunohistochemistry analysis of CD146 in paraffin-embedded mouse lung using CD146 Rabbit mAb (A24343) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of CD146 in paraffin-embedded mouse testis using CD146 Rabbit mAb (A24343) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry analysis of CD146 in paraffin-embedded rat lung using CD146 Rabbit mAb (A24343) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Flow CytoMetry - CD146 Rabbit mAb (A24343)

Flow cytometry:1X10^6 CTLL-2 cells (negative control, Left) and bEnd.3 cells (Right) were surface-stained with CD146 Rabbit mAb(A24343, 2μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2μg/mL, blue line), followed by Alexa Fluor®647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 bEnd.3 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2μg/mL, left) or CD146 Rabbit mAb(A24343, 2μg/mL, right).

Overview

Product name	CD146 Rabbit mAb
Catalog No.	A24343
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC62356

Background

Predicted to be involved in angiogenesis and positive regulation of cell migration. Predicted to act upstream of or within heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules. Predicted to be located in external side of plasma membrane; extracellular space; and nucleus. Predicted to be active in plasma membrane. Is expressed in several structures, including adipose tissue; genitourinary system; gut; nervous system; and respiratory system. Orthologous to human MCAM (melanoma cell adhesion molecule).

Immunogen information

Immunogen	Recombinant Protein corresponding to a sequence within amino acids 24-563 of mouse CD146 (NP_075548.2).
Sequence	VPGEEKQPVPTPDLVEAEVGSTALLKCGPSRASGNFSQVDWFLIHKERQILIFRVHQGKGQREPGEYEHRLSLQDSVATLALSHVTPHDERMFLCKSKRPRLQDHYVELQVFKAPEEPTIQANVVGIHVDRQELREVATCVGRNGYPIPQVLWYKNSLPLQEEENRVHIQSSQIVESSGLYTLKSVLSARLVKEDKDAQFYCELSYRLPSGNHMKESKEVTVPVFYPAEKVWVEVEPVGLLKEGDHVTIRCLTDGNPQPHFTINKKDPSTGEMEEESTDENGLLSLEPAEKHHSGLYQCQSLDLETTITLSSDPLELLVNYVSDVQVNPTAPEVQEGESLTLTCEAESNQDLEFEWLRDKTGQLLGKGPVLQLNNVRREAGGRYLCMASVPRVPGLNRTQLVSVGIFGSPWMALKERKVWVQENAVLNLSCEASGHPQPTISWNVNGSATEWNPDPQTVVSTLNVLVTPELLETGAECTASNSLGSNTTTIVLKLVTLTTLIPDSSQTTGLSTLTVSPHTRANSTSTEKKLPQPESKGVV
Gene ID
Swiss Prot
Synonyms	CD146; CD149; Muc18; s-endo; 1-gicerin; s-gicerin
Calculated MW	71kDa
Observed MW	100kDa, 110kDa, 120kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 FC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Flow Cytometry
Positive samples	bEnd.3, mouse skin, rat lung, rat uterus
Cellular location	Membrane
Customer validation	FC(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

CD146 Rabbit mAb images

Western blot - CD146 Rabbit mAb (A24343)

Immunohistochemistry - CD146 Rabbit mAb (A24343)

Flow CytoMetry - CD146 Rabbit mAb (A24343)

Flow cytometry:1X10^6 bEnd.3 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2μg/mL, left) or CD146 Rabbit mAb(A24343, 2μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.