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CD18 Rabbit mAb (A25810)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CD18 Rabbit mAb (A25810)

Western blot analysis of various lysates using CD18 Rabbit mAb (A25810) at 1:1500 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - CD18 Rabbit mAb (A25810)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using CD18 Rabbit mAb (A25810) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD18 Rabbit mAb (A25810)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD18 Rabbit mAb (A25810) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD18 Rabbit mAb (A25810)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using CD18 Rabbit mAb (A25810) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD18 Rabbit mAb (A25810)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using CD18 Rabbit mAb (A25810) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD18 Rabbit mAb (A25810)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using CD18 Rabbit mAb (A25810) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD18 Rabbit mAb (A25810)

Confocal imaging of paraffin-embedded Mouse thymus tissue using CD18 Rabbit mAb (A25810, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameCD18 Rabbit mAb
Catalog No.A25810
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC67171
This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human CD18 (NP_000202.3).
SequenceMLGLRPPLLALVGLLSLGCVLSQECTKFKVSSCRECIESGPGCTWCQKLNFTGPGDPDSIRCDTRPQLLMRGCAADDIMDPTSLAETQEDHNGGQKQLSP
Gene ID
Swiss Prot
SynonymsLAD; CD18; MF17; MFI7; LCAMB; LFA-1; MAC-1
Calculated MW85kDa
Observed MW90-95kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:800 - 1:8000
  • IF/ICC 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse spleen, Rat spleen
Cellular locationMembrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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ABclonal:Western blot - CD18 Rabbit mAb (A25810)}

Western blot - CD18 Rabbit mAb (A25810)

Western blot analysis of various lysates using CD18 Rabbit mAb (A25810) at 1:1500 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - CD18 Rabbit mAb (A25810)}

Immunohistochemistry - CD18 Rabbit mAb (A25810)

Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using CD18 Rabbit mAb (A25810) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD18 Rabbit mAb (A25810)}

Immunohistochemistry - CD18 Rabbit mAb (A25810)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD18 Rabbit mAb (A25810) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD18 Rabbit mAb (A25810)}

Immunohistochemistry - CD18 Rabbit mAb (A25810)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using CD18 Rabbit mAb (A25810) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD18 Rabbit mAb (A25810)}

Immunohistochemistry - CD18 Rabbit mAb (A25810)

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using CD18 Rabbit mAb (A25810) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD18 Rabbit mAb (A25810)}

Immunohistochemistry - CD18 Rabbit mAb (A25810)

Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using CD18 Rabbit mAb (A25810) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - CD18 Rabbit mAb (A25810)}

Immunofluorescence - CD18 Rabbit mAb (A25810)

Confocal imaging of paraffin-embedded Mouse thymus tissue using CD18 Rabbit mAb (A25810, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

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