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CD305/LAIR1 Rabbit mAb (A23775)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of various lysates, using CD305/LAIR1 Rabbit mAb (A23775) at 1:2000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Flow CytoMetry - CD305/LAIR1 Rabbit mAb (A23775)

Flow cytometry: 1X10^6 K-562 cells(negative control, left) and THP-1 cells (right) were surface-stained with ABflo® 647 Rabbit anti-CD305/LAIR1 Rabbit mAb(A23775, 2 μg/mL, orange line)or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line).Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 THP-1 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or CD305/LAIR1 Rabbit mAb(A23775, 2 μg/mL, right).

Overview

Product name	CD305/LAIR1 Rabbit mAb
Catalog No.	A23775
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61434

Background

The protein encoded by this gene is an inhibitory receptor found on peripheral mononuclear cells, including natural killer cells, T cells, and B cells. Inhibitory receptors regulate the immune response to prevent lysis of cells recognized as self. The gene is a member of both the immunoglobulin superfamily and the leukocyte-associated inhibitory receptor family. The gene maps to a region of 19q13.4 called the leukocyte receptor cluster, which contains at least 29 genes encoding leukocyte-expressed receptors of the immunoglobulin superfamily. The encoded protein has been identified as an anchor for tyrosine phosphatase SHP-1, and may induce cell death in myeloid leukemias. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-163 of human CD305/LAIR1(NP_002278.2).
Sequence	EDLPRPSISAEPGTVIPLGSHVTFVCRGPVGVQTFRLERDSRSTYNDTEDVSQASPSESEARFRIDSVREGNAGLYRCIYYKPPKWSEQSDYLELLVKESSGGPDSPDTEPGSSAGPTQRPSDNSHNEHAPASQGLKAEH
Gene ID
Swiss Prot
Synonyms	LAIR1; CD305; LAIR-1; leukocyte-associated immunoglobulin-like receptor 1; CD305/LAIR1
Calculated MW	23kDa/29kDa/31kDa
Observed MW	40-50kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 FC 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Flow Cytometry
Positive samples	THP-1
Cellular location	Cell membrane, Single-pass type I membrane protein

Documents

Certificate of Compliance

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Research Area

연구면역학 & 염증CDs

CD305/LAIR1 Rabbit mAb images

Western blot - CD305/LAIR1 Rabbit mAb (A23775)

Flow CytoMetry - CD305/LAIR1 Rabbit mAb (A23775)

Flow cytometry:1X10^6 THP-1 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or CD305/LAIR1 Rabbit mAb(A23775, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.