제품 > 항체 > 단일클론항체(mAb)

CD38 Rabbit mAb (A23037)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of various lysates, using CD38 Rabbit mAb (A23037) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - CD38 Rabbit mAb (A23037)

Confocal imaging of Mouse splenocytes cells using CD38 Rabbit mAb (A23037, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - CD38 Rabbit mAb (A23037)

Flow cytometry: 1X10^6 C2C12 cells (negative control, left) and C57/BL6 splenocytes (right) were surface-stained with CD38 Rabbit mAb (A23037, 2 μg/mL, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, blue line), followed by FITC conjugated goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57/BL6 splenocytes cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, left) or CD38 Rabbit mAb (A23037, 2 μg/mL, right).

Overview

Product name	CD38 Rabbit mAb
Catalog No.	A23037
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC59835

Background

This gene encodes a non-lineage-restricted, type II transmembrane glycoprotein that synthesizes and hydrolyzes cyclic adenosine 5'-diphosphate-ribose, an intracellular calcium ion mobilizing messenger. The release of soluble protein and the ability of membrane-bound protein to become internalized indicate both extracellular and intracellular functions for the protein. This protein has an N-terminal cytoplasmic tail, a single membrane-spanning domain, and a C-terminal extracellular region with four N-glycosylation sites. Knockout mice deficient for this gene display altered humoral immune responses. In addition, knockout mice exhibit higher locomotor activity and defects in nurturing and social behaviors.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 45-304 of mouse CD38 (NP_031672.2).
Sequence	LRPRSLLVWTGEPTTKHFSDIFLGRCLIYTQILRPEMRDQNCQEILSTFKGAFVSKNPCNITREDYAPLVKLVTQTIPCNKTLFWSKSKHLAHQYTWIQGKMFTLEDTLLGYIADDLRWCGDPSTSDMNYVSCPHWSENCPNNPITVFWKVISQKFAEDACGVVQVMLNGSLREPFYKNSTFGSVEVFSLDPNKVHKLQAWVMHDIEGASSNACSSSSLNELKMIVQKRNMIFACVDNYRPARFLQCVKNPEHPSCRLNT
Gene ID
Swiss Prot
Synonyms	I-19; ADPRC 1; Cd38-rs1; CD38
Calculated MW	34kDa
Observed MW	52kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IF/ICC 1:50 - 1:200 FC 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Flow Cytometry
Positive samples	Mouse spleen, Mouse colon
Cellular location	Membrane, Single-pass type II membrane protein.

Documents

Certificate of Compliance

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Research Area

CD38 Rabbit mAb images

Western blot - CD38 Rabbit mAb (A23037)

Immunofluorescence - CD38 Rabbit mAb (A23037)

Flow CytoMetry - CD38 Rabbit mAb (A23037)

Flow cytometry: 1X10^6 C57/BL6 splenocytes cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 2 μg/mL, left) or CD38 Rabbit mAb (A23037, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.