CD44 Rabbit mAb (A24605)

Western blot analysis of lysates from C2C12 cells, using CD44 Rabbit mAb (A24605) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Confocal imaging of NIH/3T3 cells using CD44 Rabbit mAb (A24605, dilution 1:200)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Flow cytometry: 1X10^6 CHO cells (negative control, left) and EL4 cells (right) were surface-stained with CD44 Rabbit mAb (A24605, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 CHO cells (negative control, left) and C57BL/6 splenocytes (right) were surface-stained with CD44 Rabbit mAb (A24605, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 EL4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or CD44 Rabbit mAb (A24605, 2 μg/mL, right).

Flow cytometry: 1X10^6 C57BL/6 splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or CD44 Rabbit mAb (A24605, 2 μg/mL, right).