제품 > 항체 > 단일클론항체(mAb)

CD45 Rabbit mAb (A23549)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Western blot analysis of various lysates, using CD45 Rabbit mAb (A23549) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 120s.

ABclonal:Immunohistochemistry - CD45 Rabbit mAb (A23549)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using CD45 Rabbit mAb (A23549) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CD45 Rabbit mAb (A23549)

Immunofluorescence analysis of RAW264.7 cells using CD45 Rabbit mAb (A23549) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - CD45 Rabbit mAb (A23549)

Flow cytometry: 1X10^6 C2C12 cells (negative control, left) and C57BL/6 mouse Splenocytes (right) were surface-stained with CD45 Rabbit mAb (A23549, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or CD45 Rabbit mAb (A23549, 2 μg/mL, right).

Overview

Product name	CD45 Rabbit mAb
Catalog No.	A23549
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC60962

Background

Enables several functions, including heparan sulfate proteoglycan binding activity; heparin binding activity; and protein tyrosine phosphatase activity. Involved in several processes, including lymphocyte differentiation; positive regulation of macromolecule metabolic process; and regulation of signal transduction. Acts upstream of or within several processes, including lymphocyte differentiation; positive regulation of lymphocyte activation; and regulation of protein phosphorylation. Located in external side of plasma membrane; focal adhesion; and membrane raft. Is expressed in several structures, including 3rd branchial arch; alimentary system; cardiovascular system; hemolymphoid system; and placenta. Used to study systemic lupus erythematosus. Human ortholog(s) of this gene implicated in hepatitis C; multiple sclerosis; severe combined immunodeficiency; and severe combined immunodeficiency, autosomal recessive, T cell-negative, B cell-positive, Nk cell-positive. Orthologous to human PTPRC (protein tyrosine phosphatase receptor type C).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 26-566 of mouse CD45 (NP_001104786.2)
Sequence	QTPTPSDELSTTENALLLPQSDPLPARTTESTPPSISERGNGSSETTYHPGVLSTLLPHLSPQPDSQTPSAGGADTQTFSSQADNPTLTPAPGGGTDPPGVPGERTVPGTIPADTAFPVDTPSLARNSSAASPTHTSNVSTTDISSGASLTTLTPSTLGLASTDPPSTTIATTTKQTCAAMFGNITVNYTYESSNQTFKADLKDVQNAKCGNEDCENVLNNLEECSQIKNISVSNDSCAPATTIDLYVPPGTDKFSLHDCTPKEKANTSICLEWKTKNLDFRKCNSDNISYVLHCEPENNTKCIRRNTFIPERCQLDNLRAQTNYTCVAEILYRGVKLVKNVINVQTDLGIPETPKPSCGDPAARKTLVSWPEPVSKPESASKPHGYVLCYKNNSEKCKSLPNNVTSFEVESLKPYKYYEVSLLAYVNGKIQRNGTAEKCNFHTKADRPDKVNGMKTSRPTDNSINVTCGPPYETNGPKTFYILVVRSGGSFVTKYNKTNCQFYVDNLYYSTDYEFLVSFHNGVYEGDSVIRNESTNFNAK
Gene ID
Swiss Prot
Synonyms	loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4; CD45
Calculated MW	145KDa
Observed MW	180-250kDa

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:200 - 1:800 IF 1:100 - 1:800 FC 1:100 - 1:500 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Flow Cytometry
Positive samples	RAW264.7, Mouse spleen, Mouse thymus
Cellular location	Cell membrane.
Customer validation	FC(Mus musculus) IHC(Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

CD45 Rabbit mAb images

Western blot - CD45 Rabbit mAb (A23549)

Immunohistochemistry - CD45 Rabbit mAb (A23549)

Immunofluorescence - CD45 Rabbit mAb (A23549)

Flow CytoMetry - CD45 Rabbit mAb (A23549)

Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or CD45 Rabbit mAb (A23549, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.