CD62E Rabbit mAb (A26341)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with CD62E using CD62E Rabbit mAb (A26341) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 20 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
.Exposure time: 30s.

Confocal imaging of 293T cells transfected with CD62E using CD62E Rabbit mAb (A26341, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Flow cytometry: 1X10^6 CHO cells (negative control, left) and CHO (Transfection, right) cells were surface-stained with CD62E Rabbit mAb (A26341, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by PE Goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 CHO (Transfection) cells were surface-stained with Rabbit IgG isotype control (AC042, 2 μg/mL, left) or CD62E Rabbit mAb (A26341, 2 μg/mL, right), followed by PE Goat anti-Rabbit pAb staining.

Flow cytometry: 1X10^6 bEnd.3 cells (right) were surface-stained with CD62E Rabbit mAb (A26341, 2 μg/mL, orange line) or Rabbit IgG isotype control (AC042, 2 μg/mL, blue line), followed by PE Goat anti-Rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).