제품 > 항체 > 단일클론항체(mAb)

CDA Rabbit mAb (A24610)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Immunohistochemistry analysis of CDA in paraffin-embedded human spleen using CDA Rabbit mAb (A24610) at dilution of 1:1000 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Flow CytoMetry - CDA Rabbit mAb (A24610)

Flow cytometry: 1X10^6 HEL cells (negative control, left) and HeLa cells (right) were intracellularly-stained with CDA Rabbit mAb (A24610, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by ABflo® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 HeLa cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or CDA Rabbit mAb (A24610, 2 μg/mL, right).

Overview

Product name	CDA Rabbit mAb
Catalog No.	A24610
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63546

Background

This gene encodes an enzyme involved in pyrimidine salvaging. The encoded protein forms a homotetramer that catalyzes the irreversible hydrolytic deamination of cytidine and deoxycytidine to uridine and deoxyuridine, respectively. It is one of several deaminases responsible for maintaining the cellular pyrimidine pool. Mutations in this gene are associated with decreased sensitivity to the cytosine nucleoside analogue cytosine arabinoside used in the treatment of certain childhood leukemias.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-146 of human CDA(NP_001776.1).
Sequence	MAQKRPACTLKPECVQQLLVCSQEAKKSAYCPYSHFPVGAALLTQEGRIFKGCNIENACYPLGICAERTAIQKAVSEGYKDFRAIAIASDMQDDFISPCGACRQVMREFGTNWPVYMTKPDGTYIVMTVQELLPSSFGPEDLQKTQ
Gene ID
Swiss Prot
Synonyms	CDD; CDA
Calculated MW	16kDa
Observed MW	16kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IHC-P 1:500 - 1:1000 FC (intra) 1:50- 1:100
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Immunohistochemistry
Positive samples	HeLa, PC-3, U-937
Cellular location	Cytosol, extracellular region

Documents

Certificate of Compliance

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Research Area

CDA Rabbit mAb images

Immunohistochemistry - CDA Rabbit mAb (A24610)

Flow CytoMetry - CDA Rabbit mAb (A24610)

Flow cytometry: 1X10^6 HeLa cells were intracellularly-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or CDA Rabbit mAb (A24610, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.