제품 > 항체 > 단일클론항체(mAb)

CDC23 Rabbit mAb (A6025)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of extracts of various cell lines, using CDC23 Rabbit mAb (A6025) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - CDC23 Rabbit mAb (A6025)

Immunofluorescence analysis of HeLa cells using CDC23 Rabbit mAb (A6025) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	CDC23 Rabbit mAb
Catalog No.	A6025
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2133

Background

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 498-597 of human CDC23 (Q9UJX2).
Sequence	YSCGEIVEHLEESTAFRYLAQYYFKCKLWDEASTCAQKCCAFNDTREEGKALLRQILQLRNQGETPTTEVPAPFFLPASLSANNTPTRRVSPLNLSSVTP
Gene ID
Swiss Prot
Synonyms	APC8; CUT23; ANAPC8
Calculated MW	69kDa
Observed MW	69kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, SKOV3, Mouse lung, Mouse brain, Mouse spleen, Rat brain
Cellular location	cytoplasm, cytosol, nucleoplasm
Customer validation	WB(Sus scrofa) RIP(Sus scrofa)