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CDK19 Rabbit pAb (A16109)

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Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CDK19 Rabbit pAb (A16109)

Western blot analysis of lysates from DU145 cells, using CDK19 Rabbit pAb (A16109) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - CDK19 Rabbit pAb (A16109)

Western blot analysis of lysates from Mouse brain, using CDK19 Rabbit pAb (A16109) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - CDK19 Rabbit pAb (A16109)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CDK19 Rabbit pAb (A16109) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDK19 Rabbit pAb (A16109)

Immunohistochemistry analysis of paraffin-embedded Human colon using CDK19 Rabbit pAb (A16109) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDK19 Rabbit pAb (A16109)

Immunohistochemistry analysis of paraffin-embedded Mouse stomach using CDK19 Rabbit pAb (A16109) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDK19 Rabbit pAb (A16109)

Immunohistochemistry analysis of paraffin-embedded Mouse testis using CDK19 Rabbit pAb (A16109) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CDK19 Rabbit pAb (A16109)

Immunohistochemistry analysis of paraffin-embedded Rat testis using CDK19 Rabbit pAb (A16109) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameCDK19 Rabbit pAb
Catalog No.A16109
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is one of the components of the Mediator co-activator complex. The Mediator complex is a multi-protein complex required for transcriptional activation by DNA binding transcription factors of genes transcribed by RNA polymerase II. The protein encoded by this gene is similar to cyclin-dependent kinase 8 which can also be a component of the Mediator complex. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 250-350 of human CDK19 (XP_005266928.1).
SequenceQQQNQHQQPTAPPQQAAAPPQAPPPQQNSTQTNGTAGGAGAGVGGTGAGLQHSQDSSLNQVPPNKKPRLGPSGANSGGPVMPSDYQHSSSRLNYQSSVQGS
Gene ID
Swiss Prot
SynonymsCDK11; DEE87; CDC2L6; EIEE87; bA346C16.3; CDK19
Calculated MW57kDa
Observed MW57kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesDU145, Mouse brain
Cellular locationcytosol, nucleoplasm, nucleus, perinuclear region of cytoplasm.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - CDK19 Rabbit pAb (A16109)}

    Western blot - CDK19 Rabbit pAb (A16109)

    Western blot analysis of lysates from DU145 cells, using CDK19 Rabbit pAb (A16109) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Western blot - CDK19 Rabbit pAb (A16109)}

    Western blot - CDK19 Rabbit pAb (A16109)

    Western blot analysis of lysates from Mouse brain, using CDK19 Rabbit pAb (A16109) at 1:500 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunohistochemistry - CDK19 Rabbit pAb (A16109)}

    Immunohistochemistry - CDK19 Rabbit pAb (A16109)

    Immunohistochemistry analysis of paraffin-embedded Human breast cancer using CDK19 Rabbit pAb (A16109) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - CDK19 Rabbit pAb (A16109)}

    Immunohistochemistry - CDK19 Rabbit pAb (A16109)

    Immunohistochemistry analysis of paraffin-embedded Human colon using CDK19 Rabbit pAb (A16109) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - CDK19 Rabbit pAb (A16109)}

    Immunohistochemistry - CDK19 Rabbit pAb (A16109)

    Immunohistochemistry analysis of paraffin-embedded Mouse stomach using CDK19 Rabbit pAb (A16109) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - CDK19 Rabbit pAb (A16109)}

    Immunohistochemistry - CDK19 Rabbit pAb (A16109)

    Immunohistochemistry analysis of paraffin-embedded Mouse testis using CDK19 Rabbit pAb (A16109) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
    ABclonal:Immunohistochemistry - CDK19 Rabbit pAb (A16109)}

    Immunohistochemistry - CDK19 Rabbit pAb (A16109)

    Immunohistochemistry analysis of paraffin-embedded Rat testis using CDK19 Rabbit pAb (A16109) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

    * For research use only. Not for therapeutic or diagnostic purposes.