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CFAP53 Rabbit pAb (A16607)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CFAP53 Rabbit pAb (A16607)

Western blot analysis of various lysates using CFAP53 Rabbit pAb (A16607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - CFAP53 Rabbit pAb (A16607)

Immunofluorescence analysis of HeLa cells using CFAP53 Rabbit pAb (A16607) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCFAP53 Rabbit pAb
Catalog No.A16607
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene belongs to the CFAP53 family. It was found to be differentially expressed by the ciliated cells of frog epidermis and in skin fibroblasts from human. Mutations in this gene are associated with visceral heterotaxy-6, which implicates this gene in determination of left-right asymmetric patterning.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CFAP53 (NP_659457.2).
SequenceMYSQRFGTVQREVKGPTPKVVIVRSKPPKGQGAEHHLERIRRSHQKHNAILASIKSSERDRLKAEWDQHNDCKILDSLVRARIKDAVQGFIINIEERRNKLRELLALEENEYFTEMQLKKETIEEKKDRMREKTKLLKEKNEKERQDFVAEKLDQQFRERCEELRVELLSIHQKKVCEERKAQIAFNEELSRQKLVEEQMFSKLWEEDRLAKEKREAQEARRQKELMENTRLGLNAQITSIKAQRQATQLLKEEEARLVESNNAQIKHENEQDMLKKQKAKQETRTILQKALQERIEHIQ
Gene ID
Swiss Prot
SynonymsHTX6; CCDC11; CFAP53
Calculated MW62kDa
Observed MW62kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMouse testis, Rat testis
Cellular locationCell projection, cilium
Customer validation

IF(Mus musculus)

Documents

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    ABclonal:Western blot - CFAP53 Rabbit pAb (A16607)}

    Western blot - CFAP53 Rabbit pAb (A16607)

    Western blot analysis of various lysates using CFAP53 Rabbit pAb (A16607) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - CFAP53 Rabbit pAb (A16607)}

    Immunofluorescence - CFAP53 Rabbit pAb (A16607)

    Immunofluorescence analysis of HeLa cells using CFAP53 Rabbit pAb (A16607) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using A16607? Please let us know so that we can cite the reference in this datasheet.