제품 > 항체 > 다중클론항체(pAb)

CHCHD3 Rabbit pAb (A8584)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using CHCHD3 Rabbit pAb (A8584) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 40s.

ABclonal:Immunofluorescence - CHCHD3 Rabbit pAb (A8584)

Confocal immunofluorescence analysis of U2OS cells using CHCHD3 Rabbit pAb (A8584) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - CHCHD3 Rabbit pAb (A8584)

Immunoprecipitation analysis of 600 μg extracts of Mouse liver cells using 3 μg CHCHD3 antibody (A8584). Western blot was performed from the immunoprecipitate using CHCHD3 antibody (A8584) at a dilution of 1:1000.

Overview

Product name	CHCHD3 Rabbit pAb
Catalog No.	A8584
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene is an inner mitochondrial membrane scaffold protein. Absence of the encoded protein affects the structural integrity of mitochondrial cristae and leads to reductions in ATP production, cell growth, and oxygen consumption. This protein is part of the mitochondrial contact site and cristae organizing system (MICOS). Several transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-227 of human CHCHD3 (NP_060282.1).
Sequence	MGGTTSTRRVTFEADENENITVVKGIRLSENVIDRMKESSPSGSKSQRYSGAYGASVSDEELKRRVAEELALEQAKKESEDQKRLKQAKELDRERAAANEQLTRAILRERICSEEERAKAKHLARQLEEKDRVLKKQDAFYKEQLARLEERSSEFYRVTTEQYQKAAEEVEAKFKRYESHPVCADLQAKILQCYRENTHQTLKCSALATQYMHCVNHAKQSMLEKGG
Gene ID
Swiss Prot
Synonyms	Mic19; MINOS3; MICOS19; PPP1R22; CHCHD3
Calculated MW	26kDa
Observed MW	30kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	A-549, HeLa, A-431, Jurkat, HepG2, mouse kidney, mouse liver, rat heart, rat kidney, Mouse kidney, Mouse liver, Mouse heart
Cellular location	Cytoplasm, Intermembrane side, Lipid-anchor, Mitochondrion, Mitochondrion inner membrane, Nucleus
Customer validation	WB(Homo sapiens, Mus musculus)