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CHDH Rabbit mAb (A25843)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CHDH Rabbit mAb (A25843)

Western blot analysis of various lysates using CHDH Rabbit mAb (A25843) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - CHDH Rabbit mAb (A25843)

Western blot analysis of various lysates using CHDH Rabbit mAb (A25843) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Jurkat
Exposure time: 60s.

ABclonal:Immunohistochemistry - CHDH Rabbit mAb (A25843)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using CHDH Rabbit mAb (A25843) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CHDH Rabbit mAb (A25843)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CHDH Rabbit mAb (A25843) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CHDH Rabbit mAb (A25843)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using CHDH Rabbit mAb (A25843) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CHDH Rabbit mAb (A25843)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using CHDH Rabbit mAb (A25843) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - CHDH Rabbit mAb (A25843)

Confocal imaging of paraffin-embedded Rat kidney tissue using CHDH Rabbit mAb (A25843, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

Overview

Product nameCHDH Rabbit mAb
Catalog No.A25843
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC67739
The protein encoded by this gene is a choline dehydrogenase that localizes to the mitochondrion. Variations in this gene can affect susceptibility to choline deficiency. A few transcript variants have been found for this gene, but the full-length nature of only one has been characterized to date.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 245-594 of human CHDH (NP_060867.2)
SequenceLHPALSRTNLKAEAETLVSRVLFEGTRAVGVEYVKNGQSHRAYASKEVILSGGAINSPQLLMLSGIGNADDLKKLGIPVVCHLPGVGQNLQDHLEIYIQQACTRPITLHSAQKPLRKVCIGLEWLWKFTGEGATAHLETGGFIRSQPGVPHPDIQFHFLPSQVIDHGRVPTQQEAYQVHVGPMRGTSVGWLKLRSANPQDHPVIQPNYLSTETDIEDFRLCVKLTREIFAQEALAPFRGKELQPGSHIQSDKEIDAFVRAKADSAYHPSCTCKMGQPSDPTAVVDPQTRVLGVENLRVVDASIMPSMVSGNLNAPTIMIAEKAADIIKGQPALWDKDVPVYKPRTLATQR
Gene ID
Swiss Prot
SynonymsCHDH
Calculated MW65kDa
Observed MW65kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse kidney, Rat kidney, Hep G2, 293T
Cellular locationMitochondrion inner membrane.

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CHDH Rabbit mAb (A25843)}

Western blot - CHDH Rabbit mAb (A25843)

Western blot analysis of various lysates using CHDH Rabbit mAb (A25843) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - CHDH Rabbit mAb (A25843)}

Western blot - CHDH Rabbit mAb (A25843)

Western blot analysis of various lysates using CHDH Rabbit mAb (A25843) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC): Jurkat
Exposure time: 60s.
ABclonal:Immunohistochemistry - CHDH Rabbit mAb (A25843)}

Immunohistochemistry - CHDH Rabbit mAb (A25843)

Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using CHDH Rabbit mAb (A25843) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CHDH Rabbit mAb (A25843)}

Immunohistochemistry - CHDH Rabbit mAb (A25843)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using CHDH Rabbit mAb (A25843) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CHDH Rabbit mAb (A25843)}

Immunohistochemistry - CHDH Rabbit mAb (A25843)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using CHDH Rabbit mAb (A25843) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CHDH Rabbit mAb (A25843)}

Immunohistochemistry - CHDH Rabbit mAb (A25843)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using CHDH Rabbit mAb (A25843) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - CHDH Rabbit mAb (A25843)}

Immunofluorescence - CHDH Rabbit mAb (A25843)

Confocal imaging of paraffin-embedded Rat kidney tissue using CHDH Rabbit mAb (A25843, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)