제품 > 항체 > 다중클론항체(pAb)

CKAP4 Rabbit pAb (A7777)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CKAP4 Rabbit pAb (A7777)

Western blot analysis of various lysates using CKAP4 Rabbit pAb (A7777) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - CKAP4 Rabbit pAb (A7777)

Confocal immunofluorescence analysis of U2OS cells using CKAP4 Rabbit pAb (A7777) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - CKAP4 Rabbit pAb (A7777)

Immunofluorescence analysis of U2OS cells using CKAP4 Rabbit pAb (A7777) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameCKAP4 Rabbit pAb
Catalog No.A7777
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables RNA binding activity. Located in several cellular components, including lipid droplet; nuclear speck; and rough endoplasmic reticulum.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 130-400 of human CKAP4 (NP_006816.2).
SequenceVLEEVQQVRRSHQDFSRQREELGQGLQGVEQKVQSLQATFGTFESILRSSQHKQDLTEKAVKQGESEVSRISEVLQKLQNEILKDLSDGIHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKRSQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMESDIYTEVRELVSLKQEQQAFKEAADTERLALQALTEKLLRSEESVSRLPEEIRRLEEELRQLKSDSHGPKEDGGFRHSEAFE
Gene ID
Swiss Prot
Synonymsp63; CLIMP63; CLIMP-63; ERGIC-63; CKAP4
Calculated MW66kDa
Observed MW66kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, HepG2, MCF7, NCI-H460, Mouse testis
Cellular locationCell membrane, Cytoplasm, Endoplasmic reticulum membrane, Single-pass type II membrane protein, cytoskeleton, perinuclear region
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - CKAP4 Rabbit pAb (A7777)}

Western blot - CKAP4 Rabbit pAb (A7777)

Western blot analysis of various lysates using CKAP4 Rabbit pAb (A7777) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - CKAP4 Rabbit pAb (A7777)}

Immunofluorescence - CKAP4 Rabbit pAb (A7777)

Confocal immunofluorescence analysis of U2OS cells using CKAP4 Rabbit pAb (A7777) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - CKAP4 Rabbit pAb (A7777)}

Immunofluorescence - CKAP4 Rabbit pAb (A7777)

Immunofluorescence analysis of U2OS cells using CKAP4 Rabbit pAb (A7777) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A7777? Please let us know so that we can cite the reference in this datasheet.