제품 > 항체 > 다중클론항체(pAb)

CLTB Rabbit pAb (A8404)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CLTB Rabbit pAb (A8404)

Western blot analysis of extracts of various cell lines, using CLTB antibody (A8404) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - CLTB Rabbit pAb (A8404)

Immunofluorescence analysis of HeLa cells using CLTB Polyclonal Antibody (A8404) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameCLTB Rabbit pAb
Catalog No.A8404
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human CLTB (NP_001825.1).
SequenceMADDFGFFSSSESGAPEAAEEDPAAAFLAQQESEIAGIENDEGFGAPAGSHAAPAQPGPTSGAGSEDMGTTVNGDVFQEANGPADGYAAIAQADRLTQEPESIRKWREEQRKRLQELDAASKVTEQEWREKAKKDLEEWNQRQSEQVEKNKINNRASEEAFVKESKEETPGTEWEKVAQLCDFNPKSSKQCKDVSRLRSVLMSLKQTPLSR
Gene ID
Swiss Prot
SynonymsLCB
Calculated MW25kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesLO2, A-549, MCF7, SW480, NIH/3T3, Mouse kidney, Mouse lung
Cellular locationCytoplasmic side, Cytoplasmic vesicle membrane, Membrane, Peripheral membrane protein, coated pit

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ABclonal:Western blot - CLTB Rabbit pAb (A8404)}

Western blot - CLTB Rabbit pAb (A8404)

Western blot analysis of extracts of various cell lines, using CLTB antibody (A8404) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - CLTB Rabbit pAb (A8404)}

Immunofluorescence - CLTB Rabbit pAb (A8404)

Immunofluorescence analysis of HeLa cells using CLTB Polyclonal Antibody (A8404) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.