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CNOT1 Rabbit pAb (A5969)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - CNOT1 Rabbit pAb (A5969)

Western blot analysis of various lysates, using CNOT1 Rabbit pAb (A5969) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - CNOT1 Rabbit pAb (A5969)

Immunofluorescence analysis of L929 cells using CNOT1 Rabbit pAb (A5969) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCNOT1 Rabbit pAb
Catalog No.A5969
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables armadillo repeat domain binding activity; molecular adaptor activity; and nuclear receptor binding activity. Contributes to poly(A)-specific ribonuclease activity. Involved in several processes, including negative regulation of signal transduction; positive regulation of cytoplasmic mRNA processing body assembly; and regulation of gene expression. Located in P-body and cytosol. Part of CCR4-NOT complex. Implicated in holoprosencephaly.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2137-2376 of human CNOT1 (NP_057368.3).
SequencePRNMRLPDPFTPNLKVDMLSEINIAPRILTNFTGVMPPQFKKDLDSYLKTRSPVTFLSDLRSNLQVSNEPGNRYNLQLINALVLYVGTQAIAHIHNKGSTPSMSTITHSAHMDIFQNLAVDLDTEGRYLFLNAIANQLRYPNSHTHYFSCTMLYLFAEANTEAIQEQITRVLLERLIVNRPHPWGLLITFIELIKNPAFKFWNHEFVHCAPEIEKLFQSVAQCCMGQKQAQQVMEGTGAS
Gene ID
Swiss Prot
SynonymsNOT1; CDC39; HPE12; NOT1H; VIBOS; AD-005; CNOT1
Calculated MW267kDa
Observed MW267kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, 293T, LO2, A-549, HeLa, mouse brain
Cellular locationCytoplasm, Nucleus, P-body
Customer validation

WB(Mus musculus)

IF(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - CNOT1 Rabbit pAb (A5969)}

Western blot - CNOT1 Rabbit pAb (A5969)

Western blot analysis of various lysates, using CNOT1 Rabbit pAb (A5969) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - CNOT1 Rabbit pAb (A5969)}

Immunofluorescence - CNOT1 Rabbit pAb (A5969)

Immunofluorescence analysis of L929 cells using CNOT1 Rabbit pAb (A5969) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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