제품 > 항체 > 다중클론항체(pAb)

COMMD5 Rabbit pAb (A16518)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - COMMD5 Rabbit pAb (A16518)

Western blot analysis of various lysates, using COMMD5 Rabbit pAb (A16518) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - COMMD5 Rabbit pAb (A16518)

Immunofluorescence analysis of C6 cells using COMMD5 Rabbit pAb (A16518) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameCOMMD5 Rabbit pAb
Catalog No.A16518
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in proximal tubule morphogenesis. Located in cytosol and nucleoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human COMMD5 (NP_054785.2).
SequenceMSAVGAATPYLHHPGDSHSGRVSFLGAQLPPEVAAMARLLGDLDRSTFRKLLKFVVSSLQGEDCREAVQRLGVSANLPEEQLGALLAGMHTLLQQALRLPPTSLKPDTFRDQLQELCIPQDLVGDLASVVFGSQRPLLDSVAQQQGAWLPHVADFRWRVDVAISTSALARSLQPSVLMQLKLSDGSAYRFEVPTAKFQELRYSVALVLKEMADLEKRCERRLQD
Gene ID
Swiss Prot
SynonymsHCARG; HT002; COMMD5
Calculated MW25kDa
Observed MW30kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesMCF7, PC-3, 293F
Cellular locationCytoplasm, Nucleus.

Documents

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ABclonal:Western blot - COMMD5 Rabbit pAb (A16518)}

Western blot - COMMD5 Rabbit pAb (A16518)

Western blot analysis of various lysates, using COMMD5 Rabbit pAb (A16518) at 1:3000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - COMMD5 Rabbit pAb (A16518)}

Immunofluorescence - COMMD5 Rabbit pAb (A16518)

Immunofluorescence analysis of C6 cells using COMMD5 Rabbit pAb (A16518) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.