제품 > 항체 > 다중클론항체(pAb)

CRIP1 Rabbit pAb (A7548)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CRIP1 Rabbit pAb (A7548)

Western blot analysis of various lysates using CRIP1 Rabbit pAb (A7548) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - CRIP1 Rabbit pAb (A7548)

Western blot analysis of various lysates using CRIP1 Rabbit pAb (A7548) at 1:1000 dilution.
Secondary antibody:(AS014 ) at 1:10000 dilution.
Lysates / proteins: 25 μg per lane.
Blocking buffer: 3 % nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameCRIP1 Rabbit pAb
Catalog No.A7548
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Cysteine-rich intestinal protein (CRIP) belongs to the LIM/double zinc finger protein family, members of which include cysteine- and glycine-rich protein-1 (CSRP1; MIM 123876), rhombotin-1 (RBTN1; MIM 186921), rhombotin-2 (RBTN2; MIM 180385), and rhombotin-3 (RBTN3; MIM 180386). CRIP may be involved in intestinal zinc transport (Hempe and Cousins, 1991 [PubMed 1946385]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-77 of human CRIP1 (NP_001302.1).
SequenceMPKCPKCNKEVYFAERVTSLGKDWHRPCLKCEKCGKTLTSGGHAEHEGKPYCNHPCYAAMFGPKGFGRGGAESHTFK
Gene ID
Swiss Prot
SynonymsCRHP; CRIP; CRP1; CRP-1; CRIP1
Calculated MW9kDa
Observed MW12kDa/12 kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHeLa, PC-3, OVCAR-3, Mouse lung, Mouse ovary
Cellular locationcytoplasm.
Customer validation

WB(Homo sapiens)

Documents

Certificate of Compliance

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    ABclonal:Western blot - CRIP1 Rabbit pAb (A7548)}

    Western blot - CRIP1 Rabbit pAb (A7548)

    Western blot analysis of various lysates using CRIP1 Rabbit pAb (A7548) at 1:1000 dilution.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.
    ABclonal:Western blot - CRIP1 Rabbit pAb (A7548)}

    Western blot - CRIP1 Rabbit pAb (A7548)

    Western blot analysis of various lysates using CRIP1 Rabbit pAb (A7548) at 1:1000 dilution.
    Secondary antibody:(AS014 ) at 1:10000 dilution.
    Lysates / proteins: 25 μg per lane.
    Blocking buffer: 3 % nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

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