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CTSS Rabbit pAb (A13482)

Publications (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - CTSS Rabbit pAb (A13482)

Western blot analysis of various lysates using CTSS Rabbit pAb (A13482) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - CTSS Rabbit pAb (A13482)

Western blot analysis of lysates from Rat brain, using CTSS Rabbit pAb (A13482) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - CTSS Rabbit pAb (A13482)

Immunohistochemistry analysis of paraffin-embedded Human mammary gland using CTSS Rabbit pAb (A13482) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - CTSS Rabbit pAb (A13482)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using CTSS Rabbit pAb (A13482) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product nameCTSS Rabbit pAb
Catalog No.A13482
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The preproprotein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that participates in the degradation of antigenic proteins to peptides for presentation on MHC class II molecules. The mature protein cleaves the invariant chain of MHC class II molecules in endolysosomal compartments and enables the formation of antigen-MHC class II complexes and the proper display of extracellular antigenic peptides by MHC-II. The mature protein also functions as an elastase over a broad pH range. When secreted from cells, this protein can remodel components of the extracellular matrix such as elastin, collagen, and fibronectin. This gene is implicated in the pathology of many inflammatory and autoimmune diseases and, given its elastase activity, plays a significant role in some pulmonary diseases. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 115-331 of human CTSS (NP_004070.3).
SequenceLPDSVDWREKGCVTEVKYQGSCGACWAFSAVGALEAQLKLKTGKLVSLSAQNLVDCSTEKYGNKGCNGGFMTTAFQYIIDNKGIDSDASYPYKAMDQKCQYDSKYRAATCSKYTELPYGREDVLKEAVANKGPVSVGVDARHPSFFLYRSGVYYEPSCTQNVNHGVLVVGYGDLNGKEYWLVKNSWGHNFGEEGYIRMARNKGNHCGIASFPSYPEI
Gene ID
Swiss Prot
SynonymsCTSS
Calculated MW37kDa
Observed MW25kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesTHP-1, HL-60, Mouse liver, Rat brain
Cellular locationLysosome.
Customer validation

IHC(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

ABclonal:Western blot - CTSS Rabbit pAb (A13482)}

Western blot - CTSS Rabbit pAb (A13482)

Western blot analysis of various lysates using CTSS Rabbit pAb (A13482) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - CTSS Rabbit pAb (A13482)}

Western blot - CTSS Rabbit pAb (A13482)

Western blot analysis of lysates from Rat brain, using CTSS Rabbit pAb (A13482) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - CTSS Rabbit pAb (A13482)}

Immunohistochemistry - CTSS Rabbit pAb (A13482)

Immunohistochemistry analysis of paraffin-embedded Human mammary gland using CTSS Rabbit pAb (A13482) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - CTSS Rabbit pAb (A13482)}

Immunohistochemistry - CTSS Rabbit pAb (A13482)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using CTSS Rabbit pAb (A13482) at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A13482? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

재조합 단백질 (1)

Secondary Antibodies (26)